TY - JOUR
T1 - Carboxylates and alcohols production in an autotrophic hydrogen-based membrane biofilm reactor
AU - Calvo, Diana C.
AU - Ontiveros-Valencia, Aura
AU - Krajmalnik-Brown, Rosa
AU - Torres, Cesar I.
AU - Rittmann, Bruce E.
N1 - Funding Information:
This study was supported by COLCIENCIAS (Fellowship for doctoral studies Francisco Jose de Caldas no 529), Bogota, Colombia, and by the National Science Foundation (Award number 1603656). The authors thank Juan Maldonado from the Microbiome Core Facility at ASU for their insights in the sequencing methods and uploading raw data, Esra Ilhan and Blake Dirks for their help in running Qiime2, Anca Delgado for her training and her insights in MCE, Sofia Esquivel‐Elizondo for her expertise in microbial ecology and MCE, and Alejandra Sanchez and Alejandro Calvo for their help in the MBfR schematic.
Funding Information:
This study was supported by COLCIENCIAS (Fellowship for doctoral studies Francisco Jose de Caldas no 529), Bogota, Colombia, and by the National Science Foundation?(Award number 1603656). The authors thank Juan Maldonado from the Microbiome Core Facility at ASU for their insights in the sequencing methods and uploading raw data, Esra Ilhan and Blake Dirks for their help in running Qiime2, Anca Delgado for her training and her insights in MCE, Sofia Esquivel-Elizondo for her expertise in microbial ecology and MCE, and Alejandra Sanchez and Alejandro Calvo for their help in the MBfR schematic.
Publisher Copyright:
© 2021 Wiley Periodicals LLC
PY - 2021/6
Y1 - 2021/6
N2 - Microbiological conversion of CO2 into biofuels and/or organic industrial feedstock is an excellent carbon-cycling strategy. Here, autotrophic anaerobic bacteria in the membrane biofilm reactor (MBfR) transferred electrons from hydrogen gas (H2) to inorganic carbon (IC) and produced organic acids and alcohols. We systematically varied the H2-delivery, the IC concentration, and the hydraulic retention time in the MBfR. The relative availability of H2 versus IC was the determining factor for enabling microbial chain elongation (MCE). When the H2:IC mole ratio was high (>2.0 mol H2/mol C), MCE was an important process, generating medium-chain carboxylates up to octanoate (C8, 9.1 ± 1.3 mM C and 28.1 ± 4.1 mmol C m−2 d−1). Conversely, products with two carbons were the only ones present when the H2:IC ratio was low (<2.0 mol H2/mol C), so that H2 was the limiting factor. The biofilm microbial community was enriched in phylotypes most similar to the well-known acetogen Acetobacterium for all conditions tested, but phylotypes closely related with families capable of MCE (e.g., Bacteroidales, Rhodocyclaceae, Alcaligenaceae, Thermoanaerobacteriales, and Erysipelotrichaceae) became important when the H2:IC ratio was high. Thus, proper management of IC availability and H2 supply allowed control over community structure and function, reflected by the chain length of the carboxylates and alcohols produced in the MBfR.
AB - Microbiological conversion of CO2 into biofuels and/or organic industrial feedstock is an excellent carbon-cycling strategy. Here, autotrophic anaerobic bacteria in the membrane biofilm reactor (MBfR) transferred electrons from hydrogen gas (H2) to inorganic carbon (IC) and produced organic acids and alcohols. We systematically varied the H2-delivery, the IC concentration, and the hydraulic retention time in the MBfR. The relative availability of H2 versus IC was the determining factor for enabling microbial chain elongation (MCE). When the H2:IC mole ratio was high (>2.0 mol H2/mol C), MCE was an important process, generating medium-chain carboxylates up to octanoate (C8, 9.1 ± 1.3 mM C and 28.1 ± 4.1 mmol C m−2 d−1). Conversely, products with two carbons were the only ones present when the H2:IC ratio was low (<2.0 mol H2/mol C), so that H2 was the limiting factor. The biofilm microbial community was enriched in phylotypes most similar to the well-known acetogen Acetobacterium for all conditions tested, but phylotypes closely related with families capable of MCE (e.g., Bacteroidales, Rhodocyclaceae, Alcaligenaceae, Thermoanaerobacteriales, and Erysipelotrichaceae) became important when the H2:IC ratio was high. Thus, proper management of IC availability and H2 supply allowed control over community structure and function, reflected by the chain length of the carboxylates and alcohols produced in the MBfR.
KW - alcohols production
KW - autotrophic anaerobic bacteria
KW - biofuel precursors
KW - carboxylates production
KW - membrane biofilm reactor
KW - microbial chain elongation
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UR - http://www.scopus.com/inward/citedby.url?scp=85103419899&partnerID=8YFLogxK
U2 - 10.1002/bit.27745
DO - 10.1002/bit.27745
M3 - Article
C2 - 33675236
AN - SCOPUS:85103419899
SN - 0006-3592
VL - 118
SP - 2338
EP - 2347
JO - Biotechnology and bioengineering
JF - Biotechnology and bioengineering
IS - 6
ER -