Carbon-nanotube-embedded hydrogel sheets for engineering cardiac constructs and bioactuators

Su Ryon Shin, Sung Mi Jung, Momen Zalabany, Keekyoung Kim, Pinar Zorlutuna, Sang Bok Kim, Mehdi Nikkhah, Masoud Khabiry, Mohamed Azize, Jing Kong, Kai Tak Wan, Tomas Palacios, Mehmet R. Dokmeci, Hojae Bae, Xiaowu Tang, Ali Khademhosseini

Research output: Contribution to journalArticle

396 Citations (Scopus)

Abstract

We engineered functional cardiac patches by seeding neonatal rat cardiomyocytes onto carbon nanotube (CNT)-incorporated photo-cross-linkable gelatin methacrylate (GelMA) hydrogels. The resulting cardiac constructs showed excellent mechanical integrity and advanced electrophysiological functions. Specifically, myocardial tissues cultured on 50 μm thick CNT-GelMA showed 3 times higher spontaneous synchronous beating rates and 85% lower excitation threshold, compared to those cultured on pristine GelMA hydrogels. Our results indicate that the electrically conductive and nanofibrous networks formed by CNTs within a porous gelatin framework are the key characteristics of CNT-GelMA leading to improved cardiac cell adhesion, organization, and cell-cell coupling. Centimeter-scale patches were released from glass substrates to form 3D biohybrid actuators, which showed controllable linear cyclic contraction/extension, pumping, and swimming actuations. In addition, we demonstrate for the first time that cardiac tissues cultured on CNT-GelMA resist damage by a model cardiac inhibitor as well as a cytotoxic compound. Therefore, incorporation of CNTs into gelatin, and potentially other biomaterials, could be useful in creating multifunctional cardiac scaffolds for both therapeutic purposes and in vitro studies. These hybrid materials could also be used for neuron and other muscle cells to create tissue constructs with improved organization, electroactivity, and mechanical integrity.

Original languageEnglish (US)
Pages (from-to)2369-2380
Number of pages12
JournalACS Nano
Volume7
Issue number3
DOIs
StatePublished - Mar 26 2013
Externally publishedYes

Fingerprint

Carbon Nanotubes
Hydrogel
gelatins
Gelatin
Hydrogels
Carbon nanotubes
Methacrylates
carbon nanotubes
engineering
Tissue
Cell adhesion
Hybrid materials
integrity
Biomaterials
Scaffolds
Neurons
Muscle
Rats
cells
Actuators

Keywords

  • bioactuator
  • carbon nanotubes
  • cardiac tissue engineering
  • gelatin
  • hydrogel

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Shin, S. R., Jung, S. M., Zalabany, M., Kim, K., Zorlutuna, P., Kim, S. B., ... Khademhosseini, A. (2013). Carbon-nanotube-embedded hydrogel sheets for engineering cardiac constructs and bioactuators. ACS Nano, 7(3), 2369-2380. https://doi.org/10.1021/nn305559j

Carbon-nanotube-embedded hydrogel sheets for engineering cardiac constructs and bioactuators. / Shin, Su Ryon; Jung, Sung Mi; Zalabany, Momen; Kim, Keekyoung; Zorlutuna, Pinar; Kim, Sang Bok; Nikkhah, Mehdi; Khabiry, Masoud; Azize, Mohamed; Kong, Jing; Wan, Kai Tak; Palacios, Tomas; Dokmeci, Mehmet R.; Bae, Hojae; Tang, Xiaowu; Khademhosseini, Ali.

In: ACS Nano, Vol. 7, No. 3, 26.03.2013, p. 2369-2380.

Research output: Contribution to journalArticle

Shin, SR, Jung, SM, Zalabany, M, Kim, K, Zorlutuna, P, Kim, SB, Nikkhah, M, Khabiry, M, Azize, M, Kong, J, Wan, KT, Palacios, T, Dokmeci, MR, Bae, H, Tang, X & Khademhosseini, A 2013, 'Carbon-nanotube-embedded hydrogel sheets for engineering cardiac constructs and bioactuators', ACS Nano, vol. 7, no. 3, pp. 2369-2380. https://doi.org/10.1021/nn305559j
Shin SR, Jung SM, Zalabany M, Kim K, Zorlutuna P, Kim SB et al. Carbon-nanotube-embedded hydrogel sheets for engineering cardiac constructs and bioactuators. ACS Nano. 2013 Mar 26;7(3):2369-2380. https://doi.org/10.1021/nn305559j
Shin, Su Ryon ; Jung, Sung Mi ; Zalabany, Momen ; Kim, Keekyoung ; Zorlutuna, Pinar ; Kim, Sang Bok ; Nikkhah, Mehdi ; Khabiry, Masoud ; Azize, Mohamed ; Kong, Jing ; Wan, Kai Tak ; Palacios, Tomas ; Dokmeci, Mehmet R. ; Bae, Hojae ; Tang, Xiaowu ; Khademhosseini, Ali. / Carbon-nanotube-embedded hydrogel sheets for engineering cardiac constructs and bioactuators. In: ACS Nano. 2013 ; Vol. 7, No. 3. pp. 2369-2380.
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