Calcium signals in neutrophils can be divided into three distinct phases

Douglas E. Chandler; Charles J. Kazilek

doi:10.1016/0167-4889(87)90204-7

Calcium signals in neutrophils can be divided into three distinct phases

Douglas E. Chandler, Charles J. Kazilek

Life Sciences, School of (SOLS)

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Rabbit neutrophils were loaded with the fluorescence probe indo-1 and cytosolic free calcium levels were monitored during chemotactic peptide (fMet-Leu-Phe) stimulation. The fMet-Leu-Phe-induced calcium signal consisted of three consecutive phases: (1) an initial peak that was independent of extracellular calcium, (2) a secondary shoulder that required extracellular calcium but was totally blocked by hyperosmolality and (3) a final plateau of elevated calcium that was dependent on extracellular calcium but insensitive to hyperosmolality.

Original language	English (US)
Pages (from-to)	175-179
Number of pages	5
Journal	BBA - Molecular Cell Research
Volume	931
Issue number	2
DOIs	https://doi.org/10.1016/0167-4889(87)90204-7
State	Published - Nov 12 1987

Keywords

(Neutrophil)
Calcium signal
Chemotactic peptide
Hyperosmolality
Lysosomal enzyme release

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/0167-4889(87)90204-7

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title = "Calcium signals in neutrophils can be divided into three distinct phases",

abstract = "Rabbit neutrophils were loaded with the fluorescence probe indo-1 and cytosolic free calcium levels were monitored during chemotactic peptide (fMet-Leu-Phe) stimulation. The fMet-Leu-Phe-induced calcium signal consisted of three consecutive phases: (1) an initial peak that was independent of extracellular calcium, (2) a secondary shoulder that required extracellular calcium but was totally blocked by hyperosmolality and (3) a final plateau of elevated calcium that was dependent on extracellular calcium but insensitive to hyperosmolality.",

keywords = "(Neutrophil), Calcium signal, Chemotactic peptide, Hyperosmolality, Lysosomal enzyme release",

author = "Chandler, {Douglas E.} and Kazilek, {Charles J.}",

note = "Funding Information: This study was supported by NSF (DCB-8407152) and NIH (1-K04-HD00619) grants.",

year = "1987",

month = nov,

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doi = "10.1016/0167-4889(87)90204-7",

language = "English (US)",

volume = "931",

pages = "175--179",

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T1 - Calcium signals in neutrophils can be divided into three distinct phases

AU - Chandler, Douglas E.

AU - Kazilek, Charles J.

N1 - Funding Information: This study was supported by NSF (DCB-8407152) and NIH (1-K04-HD00619) grants.

PY - 1987/11/12

Y1 - 1987/11/12

N2 - Rabbit neutrophils were loaded with the fluorescence probe indo-1 and cytosolic free calcium levels were monitored during chemotactic peptide (fMet-Leu-Phe) stimulation. The fMet-Leu-Phe-induced calcium signal consisted of three consecutive phases: (1) an initial peak that was independent of extracellular calcium, (2) a secondary shoulder that required extracellular calcium but was totally blocked by hyperosmolality and (3) a final plateau of elevated calcium that was dependent on extracellular calcium but insensitive to hyperosmolality.

AB - Rabbit neutrophils were loaded with the fluorescence probe indo-1 and cytosolic free calcium levels were monitored during chemotactic peptide (fMet-Leu-Phe) stimulation. The fMet-Leu-Phe-induced calcium signal consisted of three consecutive phases: (1) an initial peak that was independent of extracellular calcium, (2) a secondary shoulder that required extracellular calcium but was totally blocked by hyperosmolality and (3) a final plateau of elevated calcium that was dependent on extracellular calcium but insensitive to hyperosmolality.

KW - (Neutrophil)

KW - Calcium signal

KW - Chemotactic peptide

KW - Hyperosmolality

KW - Lysosomal enzyme release

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JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

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Calcium signals in neutrophils can be divided into three distinct phases

Abstract

Keywords

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