CADp44: A novel regulatory subunit of the 26S proteasome and the mammalian homolog of yeast Sug2p

Vernon W. Bauer, Jonathan C. Swaffield, Stephen Albert Johnston, Matthew T. Andrews

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We have identified a novel protein, CADp44, based on the analysis of cDNAs derived from the brainstem of the 13-lined ground squirrel, Spermophilus tridecemlineatus. CADp44 has an unmodified molecular mass of 44,178 Da and contains multiple functional domains, including a conserved ATPase domain (CAD) and a leucine zipper motif. We show that distinct regions of the CADp44 sequence are identical to a set of peptides prepared from a recently identified bovine protein, referred to as p42, which is found in the PA700 regulatory complex of the 26S proteasome. We also show that CADp44 is the functional homolog of the newly characterized Sug2 protein from the budding yeast, Saccharomyces cerevisiae. Consistent with its role as a component of the 26S proteasome, CADp44 mRNA is found in all ground squirrel tissues examined. Evolutionary relationships based on sequence analysis show that both CADp44 and yeast Sug2p are distinct from the other five CAD ATPases found in the PA700, and together comprise the sixth and newest CAD subunit of the regulatory complex of the 26S proteasome.

Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
JournalGene
Volume181
Issue number1-2
DOIs
StatePublished - Nov 28 1996
Externally publishedYes

Keywords

  • 26S proteasome
  • CADp44
  • Spermophilus
  • Yeast Sug2p

ASJC Scopus subject areas

  • Genetics

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