c-Ki-ras mutations in dysplastic fields and cancers in ulcerative colitis

James Chen, Carolyn Compton, Edward Cheng, Frank Fromowitz, Michael V. Viola

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A sensitive restriction fragment length polymorphism assay and DNA sequencing were used to detect c-Ki-ras mutations in 56 specimens of colonic epithelium from 18 patients with chronic ulcerative colitis. Mutations were not detected in biopsy specimens that were negative or indefinite for dysplasia. In 4 of 8 patients with high-grade dysplasia, a c-Ki-ras codon 12 or 13 mutation was detected. In three colectomy specimens, a wide area of dysplastic cells (>10 cm2) contained a specific ras mutation. In two of these specimens, an invasive cancer contained a c-Ki-ras mutation identical to that found in adjacent dysplastic epithelium. These studies indicate that mutations of c-Ki-ras may be an excellent molecular genetic marker to map dysplastic fields and invasive cancer in ulcerative colitis.

Original languageEnglish (US)
Pages (from-to)1983-1987
Number of pages5
JournalGastroenterology
Volume102
Issue number6
StatePublished - 1992
Externally publishedYes

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Ulcerative Colitis
Mutation
Neoplasms
Epithelium
Colectomy
DNA Sequence Analysis
Genetic Markers
Codon
Restriction Fragment Length Polymorphisms
Molecular Biology
Biopsy

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Chen, J., Compton, C., Cheng, E., Fromowitz, F., & Viola, M. V. (1992). c-Ki-ras mutations in dysplastic fields and cancers in ulcerative colitis. Gastroenterology, 102(6), 1983-1987.

c-Ki-ras mutations in dysplastic fields and cancers in ulcerative colitis. / Chen, James; Compton, Carolyn; Cheng, Edward; Fromowitz, Frank; Viola, Michael V.

In: Gastroenterology, Vol. 102, No. 6, 1992, p. 1983-1987.

Research output: Contribution to journalArticle

Chen, J, Compton, C, Cheng, E, Fromowitz, F & Viola, MV 1992, 'c-Ki-ras mutations in dysplastic fields and cancers in ulcerative colitis', Gastroenterology, vol. 102, no. 6, pp. 1983-1987.
Chen, James ; Compton, Carolyn ; Cheng, Edward ; Fromowitz, Frank ; Viola, Michael V. / c-Ki-ras mutations in dysplastic fields and cancers in ulcerative colitis. In: Gastroenterology. 1992 ; Vol. 102, No. 6. pp. 1983-1987.
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