c-Ki-ras mutations in dysplastic fields and cancers in ulcerative colitis

James Chen, Carolyn Compton, Edward Cheng, Frank Fromowitz, Michael V. Viola

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

A sensitive restriction fragment length polymorphism assay and DNA sequencing were used to detect c-Ki-ras mutations in 56 specimens of colonic epithelium from 18 patients with chronic ulcerative colitis. Mutations were not detected in biopsy specimens that were negative or indefinite for dysplasia. In 4 of 8 patients with high-grade dysplasia, a c-Ki-ras codon 12 or 13 mutation was detected. In three colectomy specimens, a wide area of dysplastic cells (>10 cm2) contained a specific ras mutation. In two of these specimens, an invasive cancer contained a c-Ki-ras mutation identical to that found in adjacent dysplastic epithelium. These studies indicate that mutations of c-Ki-ras may be an excellent molecular genetic marker to map dysplastic fields and invasive cancer in ulcerative colitis.

Original languageEnglish (US)
Pages (from-to)1983-1987
Number of pages5
JournalGastroenterology
Volume102
Issue number6
DOIs
StatePublished - Jun 1992
Externally publishedYes

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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