c-Jun N-terminal kinase signaling regulates events associated with both health and degeneration in motoneurons

Jason Newbern, A. Taylor, M. Robinson, M. O. Lively, C. E. Milligan

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The c-Jun N-terminal kinases (JNKs) are activated by various stimuli and are critical for neuronal development as well as for death following a stressful stimulus. Here, we have evaluated JNK activity in both healthy and dying motoneurons from developing chick embryos and found no apparent difference in overall JNK activity between the conditions, suggesting that this pathway maybe critical in both circumstances. Pharmacological inhibition of JNK in healthy motoneurons supplied with trophic support resulted in decreased mitochondrial membrane potential, neurite outgrowth, and phosphorylation of microtubule-associated protein 1B. On the other hand, in motoneurons deprived of trophic support, inhibition of JNK attenuated caspase activation, and nuclear condensation. We also examined the role of JNK's downstream substrate c-Jun in mediating these events. While c-Jun expression and phosphorylation were greater in cells supplied with trophic support as compared with those deprived, inhibition of c-Jun had no effect on nuclear condensation in dying cells or neurite outgrowth in healthy cells, suggesting that JNK's role in these events is independent of c-Jun. Together, our data underscore the dualistic nature of JNK signaling that is critical for both survival and degenerative changes in motoneurons.

Original languageEnglish (US)
Pages (from-to)680-692
Number of pages13
JournalNeuroscience
Volume147
Issue number3
DOIs
StatePublished - Jul 13 2007
Externally publishedYes

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JNK Mitogen-Activated Protein Kinases
Motor Neurons
Phosphotransferases
Health
Phosphorylation
Critical Pathways
Mitochondrial Membrane Potential
Chick Embryo
Caspases
Pharmacology
Neuronal Outgrowth

Keywords

  • apoptosis
  • cell death
  • neurite outgrowth
  • signal transduction
  • trophic factor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

c-Jun N-terminal kinase signaling regulates events associated with both health and degeneration in motoneurons. / Newbern, Jason; Taylor, A.; Robinson, M.; Lively, M. O.; Milligan, C. E.

In: Neuroscience, Vol. 147, No. 3, 13.07.2007, p. 680-692.

Research output: Contribution to journalArticle

Newbern, Jason ; Taylor, A. ; Robinson, M. ; Lively, M. O. ; Milligan, C. E. / c-Jun N-terminal kinase signaling regulates events associated with both health and degeneration in motoneurons. In: Neuroscience. 2007 ; Vol. 147, No. 3. pp. 680-692.
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