Abstract

This work demonstrates bromate (BrO3 -) reduction in a methane (CH4)-based membrane biofilm reactor (MBfR), and it documents contrasting impacts of nitrate (NO3 -) on BrO3 - reduction, as well as formation of poly-β-hydroxybutyrate (PHB), an internal C- and electron-storage material. When the electron donor, CH4, was in ample supply, NO3 - enhanced BrO3 - reduction by stimulating the growth of denitrifying bacteria (Meiothermus, Comamonadaceae, and Anaerolineaceae) able to reduce BrO3 - and NO3 - simultaneously. This was supported by increases in denitrifying enzymes (e.g., nitrate reductase, nitrite reductase, nitrous-oxide reductase, and nitric-oxide reductase) through quantitative polymerase chain reaction (qPCR) analysis and metagenomic prediction of these functional genes. When the electron donor was in limited supply, NO3 - was the preferred electron acceptor over BrO3 - due to competition for the common electron donor; this was supported by the significant oxidation of stored PHB when NO3 - was high enough to cause electron-donor limitation. Methanotrophs (e.g., Methylocystis, Methylomonas, and genera within Comamonadaceae) were implicated as the main PHB producers in the biofilms, and their ability to oxidize PHB mitigated the impacts of competition for CH4.

Original languageEnglish (US)
Pages (from-to)7024-7031
Number of pages8
JournalEnvironmental Science and Technology
Volume52
Issue number12
DOIs
StatePublished - Jun 19 2018

Fingerprint

Bromates
Hydroxybutyrates
Methane
Biofilms
Nitrates
biofilm
methane
nitrate
membrane
Membranes
electron
Electrons
Nitrite Reductases
Nitrate Reductase
Polymerase chain reaction
nitric oxide
nitrous oxide
polymerase chain reaction
nitrite
reactor

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Bromate and Nitrate Bioreduction Coupled with Poly-β-hydroxybutyrate Production in a Methane-Based Membrane Biofilm Reactor. / Lai, Chun Yu; Lv, Pan Long; Dong, Qiu Yi; Yeo, Shi Lei; Rittmann, Bruce; Zhao, He Ping.

In: Environmental Science and Technology, Vol. 52, No. 12, 19.06.2018, p. 7024-7031.

Research output: Contribution to journalArticle

Lai, Chun Yu ; Lv, Pan Long ; Dong, Qiu Yi ; Yeo, Shi Lei ; Rittmann, Bruce ; Zhao, He Ping. / Bromate and Nitrate Bioreduction Coupled with Poly-β-hydroxybutyrate Production in a Methane-Based Membrane Biofilm Reactor. In: Environmental Science and Technology. 2018 ; Vol. 52, No. 12. pp. 7024-7031.
@article{980c7a3f967d4457bf4e10fc8440ea5e,
title = "Bromate and Nitrate Bioreduction Coupled with Poly-β-hydroxybutyrate Production in a Methane-Based Membrane Biofilm Reactor",
abstract = "This work demonstrates bromate (BrO3 -) reduction in a methane (CH4)-based membrane biofilm reactor (MBfR), and it documents contrasting impacts of nitrate (NO3 -) on BrO3 - reduction, as well as formation of poly-β-hydroxybutyrate (PHB), an internal C- and electron-storage material. When the electron donor, CH4, was in ample supply, NO3 - enhanced BrO3 - reduction by stimulating the growth of denitrifying bacteria (Meiothermus, Comamonadaceae, and Anaerolineaceae) able to reduce BrO3 - and NO3 - simultaneously. This was supported by increases in denitrifying enzymes (e.g., nitrate reductase, nitrite reductase, nitrous-oxide reductase, and nitric-oxide reductase) through quantitative polymerase chain reaction (qPCR) analysis and metagenomic prediction of these functional genes. When the electron donor was in limited supply, NO3 - was the preferred electron acceptor over BrO3 - due to competition for the common electron donor; this was supported by the significant oxidation of stored PHB when NO3 - was high enough to cause electron-donor limitation. Methanotrophs (e.g., Methylocystis, Methylomonas, and genera within Comamonadaceae) were implicated as the main PHB producers in the biofilms, and their ability to oxidize PHB mitigated the impacts of competition for CH4.",
author = "Lai, {Chun Yu} and Lv, {Pan Long} and Dong, {Qiu Yi} and Yeo, {Shi Lei} and Bruce Rittmann and Zhao, {He Ping}",
year = "2018",
month = "6",
day = "19",
doi = "10.1021/acs.est.8b00152",
language = "English (US)",
volume = "52",
pages = "7024--7031",
journal = "Environmental Science & Technology",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "12",

}

TY - JOUR

T1 - Bromate and Nitrate Bioreduction Coupled with Poly-β-hydroxybutyrate Production in a Methane-Based Membrane Biofilm Reactor

AU - Lai, Chun Yu

AU - Lv, Pan Long

AU - Dong, Qiu Yi

AU - Yeo, Shi Lei

AU - Rittmann, Bruce

AU - Zhao, He Ping

PY - 2018/6/19

Y1 - 2018/6/19

N2 - This work demonstrates bromate (BrO3 -) reduction in a methane (CH4)-based membrane biofilm reactor (MBfR), and it documents contrasting impacts of nitrate (NO3 -) on BrO3 - reduction, as well as formation of poly-β-hydroxybutyrate (PHB), an internal C- and electron-storage material. When the electron donor, CH4, was in ample supply, NO3 - enhanced BrO3 - reduction by stimulating the growth of denitrifying bacteria (Meiothermus, Comamonadaceae, and Anaerolineaceae) able to reduce BrO3 - and NO3 - simultaneously. This was supported by increases in denitrifying enzymes (e.g., nitrate reductase, nitrite reductase, nitrous-oxide reductase, and nitric-oxide reductase) through quantitative polymerase chain reaction (qPCR) analysis and metagenomic prediction of these functional genes. When the electron donor was in limited supply, NO3 - was the preferred electron acceptor over BrO3 - due to competition for the common electron donor; this was supported by the significant oxidation of stored PHB when NO3 - was high enough to cause electron-donor limitation. Methanotrophs (e.g., Methylocystis, Methylomonas, and genera within Comamonadaceae) were implicated as the main PHB producers in the biofilms, and their ability to oxidize PHB mitigated the impacts of competition for CH4.

AB - This work demonstrates bromate (BrO3 -) reduction in a methane (CH4)-based membrane biofilm reactor (MBfR), and it documents contrasting impacts of nitrate (NO3 -) on BrO3 - reduction, as well as formation of poly-β-hydroxybutyrate (PHB), an internal C- and electron-storage material. When the electron donor, CH4, was in ample supply, NO3 - enhanced BrO3 - reduction by stimulating the growth of denitrifying bacteria (Meiothermus, Comamonadaceae, and Anaerolineaceae) able to reduce BrO3 - and NO3 - simultaneously. This was supported by increases in denitrifying enzymes (e.g., nitrate reductase, nitrite reductase, nitrous-oxide reductase, and nitric-oxide reductase) through quantitative polymerase chain reaction (qPCR) analysis and metagenomic prediction of these functional genes. When the electron donor was in limited supply, NO3 - was the preferred electron acceptor over BrO3 - due to competition for the common electron donor; this was supported by the significant oxidation of stored PHB when NO3 - was high enough to cause electron-donor limitation. Methanotrophs (e.g., Methylocystis, Methylomonas, and genera within Comamonadaceae) were implicated as the main PHB producers in the biofilms, and their ability to oxidize PHB mitigated the impacts of competition for CH4.

UR - http://www.scopus.com/inward/record.url?scp=85047568419&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047568419&partnerID=8YFLogxK

U2 - 10.1021/acs.est.8b00152

DO - 10.1021/acs.est.8b00152

M3 - Article

C2 - 29785845

AN - SCOPUS:85047568419

VL - 52

SP - 7024

EP - 7031

JO - Environmental Science & Technology

JF - Environmental Science & Technology

SN - 0013-936X

IS - 12

ER -