Abstract

Objectives: The capacity to properly address the worldwide incidence of infectious diseases lies in the ability to detect, prevent and effectively treat these infections. Therefore, identifying and analysing inhibitory agents are worthwhile endeavours in an era when few new classes of effective antimicrobials have been developed. The use of geological nanomaterials to heal skin infections has been evident since the earliest recorded history, and specific clay minerals may prove valuable in the treatment of bacterial diseases, including infections for which there are no effective antibiotics, such as Buruli ulcer and multidrug-resistant infections. Methods: We have subjected two iron-rich clay minerals, which have previously been used to treat Buruli ulcer patients, to broth culture testing of antibiotic-susceptible and antibiotic-resistant pathogenic bacteria to assess the feasibility of using clay minerals as therapeutic agents. Results: One specific mineral, CsAg02, demonstrated bactericidal activity against pathogenic Escherichia coli, extended-spectrum β-lactamase (ESBL) E. coli, Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa and Mycobacterium marinum, and a combined bacteriostatic/bactericidal effect against Staphylococcus aureus, penicillin-resistant S. aureus, methicillin-resistant S. aureus (MRSA) and Mycobacterium smegmatis, whereas another mineral with similar structure and bulk crystal chemistry, CsAr02, had no effect on or enhanced bacterial growth. The <0.2 ̄m fraction of CsAg02 and CsAg02 heated to 200 or 550°C retained bactericidal activity, whereas cation-exchanged CsAg02 and CsAg02 heated to 900°C no longer killed E. coli. Conclusions: Our results indicate that specific mineral products have intrinsic, heat-stable antibacterial properties, which could provide an inexpensive treatment against numerous human bacterial infections.

Original languageEnglish (US)
Pages (from-to)353-361
Number of pages9
JournalJournal of Antimicrobial Chemotherapy
Volume61
Issue number2
DOIs
StatePublished - Feb 2008

Fingerprint

Minerals
Anti-Bacterial Agents
Buruli Ulcer
Escherichia coli
Infection
Staphylococcus aureus
Mycobacterium marinum
Mycobacterium smegmatis
Salmonella enterica
Nanostructures
Methicillin-Resistant Staphylococcus aureus
Bacterial Infections
Penicillins
Pseudomonas aeruginosa
Communicable Diseases
clay
In Vitro Techniques
Cations
Therapeutics
Iron

Keywords

  • Bactericidal
  • Bacteriostatic
  • Infections
  • Nanominerals
  • Natural
  • Therapeutics

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology

Cite this

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title = "Broad-spectrum in vitro antibacterial activities of clay minerals against antibiotic-susceptible and antibiotic-resistant bacterial pathogens",
abstract = "Objectives: The capacity to properly address the worldwide incidence of infectious diseases lies in the ability to detect, prevent and effectively treat these infections. Therefore, identifying and analysing inhibitory agents are worthwhile endeavours in an era when few new classes of effective antimicrobials have been developed. The use of geological nanomaterials to heal skin infections has been evident since the earliest recorded history, and specific clay minerals may prove valuable in the treatment of bacterial diseases, including infections for which there are no effective antibiotics, such as Buruli ulcer and multidrug-resistant infections. Methods: We have subjected two iron-rich clay minerals, which have previously been used to treat Buruli ulcer patients, to broth culture testing of antibiotic-susceptible and antibiotic-resistant pathogenic bacteria to assess the feasibility of using clay minerals as therapeutic agents. Results: One specific mineral, CsAg02, demonstrated bactericidal activity against pathogenic Escherichia coli, extended-spectrum β-lactamase (ESBL) E. coli, Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa and Mycobacterium marinum, and a combined bacteriostatic/bactericidal effect against Staphylococcus aureus, penicillin-resistant S. aureus, methicillin-resistant S. aureus (MRSA) and Mycobacterium smegmatis, whereas another mineral with similar structure and bulk crystal chemistry, CsAr02, had no effect on or enhanced bacterial growth. The <0.2 ̄m fraction of CsAg02 and CsAg02 heated to 200 or 550°C retained bactericidal activity, whereas cation-exchanged CsAg02 and CsAg02 heated to 900°C no longer killed E. coli. Conclusions: Our results indicate that specific mineral products have intrinsic, heat-stable antibacterial properties, which could provide an inexpensive treatment against numerous human bacterial infections.",
keywords = "Bactericidal, Bacteriostatic, Infections, Nanominerals, Natural, Therapeutics",
author = "Shelley Haydel and Remenih, {Christine M.} and Lynda Williams",
year = "2008",
month = "2",
doi = "10.1093/jac/dkm468",
language = "English (US)",
volume = "61",
pages = "353--361",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
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}

TY - JOUR

T1 - Broad-spectrum in vitro antibacterial activities of clay minerals against antibiotic-susceptible and antibiotic-resistant bacterial pathogens

AU - Haydel, Shelley

AU - Remenih, Christine M.

AU - Williams, Lynda

PY - 2008/2

Y1 - 2008/2

N2 - Objectives: The capacity to properly address the worldwide incidence of infectious diseases lies in the ability to detect, prevent and effectively treat these infections. Therefore, identifying and analysing inhibitory agents are worthwhile endeavours in an era when few new classes of effective antimicrobials have been developed. The use of geological nanomaterials to heal skin infections has been evident since the earliest recorded history, and specific clay minerals may prove valuable in the treatment of bacterial diseases, including infections for which there are no effective antibiotics, such as Buruli ulcer and multidrug-resistant infections. Methods: We have subjected two iron-rich clay minerals, which have previously been used to treat Buruli ulcer patients, to broth culture testing of antibiotic-susceptible and antibiotic-resistant pathogenic bacteria to assess the feasibility of using clay minerals as therapeutic agents. Results: One specific mineral, CsAg02, demonstrated bactericidal activity against pathogenic Escherichia coli, extended-spectrum β-lactamase (ESBL) E. coli, Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa and Mycobacterium marinum, and a combined bacteriostatic/bactericidal effect against Staphylococcus aureus, penicillin-resistant S. aureus, methicillin-resistant S. aureus (MRSA) and Mycobacterium smegmatis, whereas another mineral with similar structure and bulk crystal chemistry, CsAr02, had no effect on or enhanced bacterial growth. The <0.2 ̄m fraction of CsAg02 and CsAg02 heated to 200 or 550°C retained bactericidal activity, whereas cation-exchanged CsAg02 and CsAg02 heated to 900°C no longer killed E. coli. Conclusions: Our results indicate that specific mineral products have intrinsic, heat-stable antibacterial properties, which could provide an inexpensive treatment against numerous human bacterial infections.

AB - Objectives: The capacity to properly address the worldwide incidence of infectious diseases lies in the ability to detect, prevent and effectively treat these infections. Therefore, identifying and analysing inhibitory agents are worthwhile endeavours in an era when few new classes of effective antimicrobials have been developed. The use of geological nanomaterials to heal skin infections has been evident since the earliest recorded history, and specific clay minerals may prove valuable in the treatment of bacterial diseases, including infections for which there are no effective antibiotics, such as Buruli ulcer and multidrug-resistant infections. Methods: We have subjected two iron-rich clay minerals, which have previously been used to treat Buruli ulcer patients, to broth culture testing of antibiotic-susceptible and antibiotic-resistant pathogenic bacteria to assess the feasibility of using clay minerals as therapeutic agents. Results: One specific mineral, CsAg02, demonstrated bactericidal activity against pathogenic Escherichia coli, extended-spectrum β-lactamase (ESBL) E. coli, Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa and Mycobacterium marinum, and a combined bacteriostatic/bactericidal effect against Staphylococcus aureus, penicillin-resistant S. aureus, methicillin-resistant S. aureus (MRSA) and Mycobacterium smegmatis, whereas another mineral with similar structure and bulk crystal chemistry, CsAr02, had no effect on or enhanced bacterial growth. The <0.2 ̄m fraction of CsAg02 and CsAg02 heated to 200 or 550°C retained bactericidal activity, whereas cation-exchanged CsAg02 and CsAg02 heated to 900°C no longer killed E. coli. Conclusions: Our results indicate that specific mineral products have intrinsic, heat-stable antibacterial properties, which could provide an inexpensive treatment against numerous human bacterial infections.

KW - Bactericidal

KW - Bacteriostatic

KW - Infections

KW - Nanominerals

KW - Natural

KW - Therapeutics

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DO - 10.1093/jac/dkm468

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VL - 61

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JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 2

ER -