Breathprints of model murine bacterial lung infections are linked with immune response

Heather Bean, Jaime Jiménez-Díaz, Jiangjiang Zhu, Jane E. Hill

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In this model study, we explored the host's contribution of breath volatiles to diagnostic secondary electrospray ionisation-mass spectrometry (SESI-MS) breathprints for acute bacterial lung infections, their correlation with the host's immune response, and their use in identifying the lung pathogen. Murine airways were exposed to Pseudomonas aeruginosa and Staphylococcus aureus bacterial cell lysates or to PBS (controls), and their breath and bronchoalveolar lavage fluid (BALF) were collected at six time points (from 6 to 120 h) after exposure. Five to six mice per treatment group and four to six mice per control group were sampled at each time. Breath volatiles were analysed using SESI-MS and the BALF total leukocytes, polymorphonuclear neutrophils, lactate dehydrogenase activity, and cytokine concentrations were quantified. Lysate exposure breathprints contain host volatiles that persist for up to 120 h; are pathogen specific; are unique from breathprints of controls, active infections and cleared infections; and are correlated with the host's immune response. Bacterial lung infections induce changes to the host's breath volatiles that are selective and specific predictors of the source of infection. Harnessing the pathogen-specific volatiles in the host's breath may provide useful information for detecting latent bacterial lung infections and managing the spread of respiratory diseases.

Original languageEnglish (US)
Pages (from-to)181-190
Number of pages10
JournalEuropean Respiratory Journal
Volume45
Issue number1
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

Bacterial Infections
Lung
Electrospray Ionization Mass Spectrometry
Bronchoalveolar Lavage Fluid
Neutrophils
Infection Control
Infection
L-Lactate Dehydrogenase
Pseudomonas aeruginosa
Staphylococcus aureus
Cytokines
Control Groups
Therapeutics

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Breathprints of model murine bacterial lung infections are linked with immune response. / Bean, Heather; Jiménez-Díaz, Jaime; Zhu, Jiangjiang; Hill, Jane E.

In: European Respiratory Journal, Vol. 45, No. 1, 01.01.2015, p. 181-190.

Research output: Contribution to journalArticle

Bean, Heather ; Jiménez-Díaz, Jaime ; Zhu, Jiangjiang ; Hill, Jane E. / Breathprints of model murine bacterial lung infections are linked with immune response. In: European Respiratory Journal. 2015 ; Vol. 45, No. 1. pp. 181-190.
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