Boveri's long experiment

Sea urchin merogones and the establishment of the role of nuclear chromosomes in development

Manfred Laubichler, Eric H. Davidson

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Theodor Boveri's major intellectual contribution was his focus on the causality of nuclear chromosomal determinants for embryological development. His initial experimental attempt to demonstrate that the character of the developing embryo is determined by nuclear rather than cytoplasmic factors was launched in 1889. The experimental design was to fertilize enucleate sea urchin eggs with sperm of another species that produces a distinguishably different embryonic morphology. Boveri's "hybrid merogone" experiment provided what he initially thought was empirical evidence for the nuclear control of development. However, for subtle reasons, the data were not interpretable and the experiment was repeated and contested. At the end of his life, Boveri was finally able to explain the technical difficulties that had beset the original experiment. However, by 1902 Boveri had carried out his famous polyspermy experiments, which provided decisive evidence for the role of nuclear chromosomal determinants in embryogenesis. Here we present the history of the hybrid merogone experiment as an important case of conceptual reasoning paired with (often difficult) experimental approaches. We then trace the further history of the merogone and normal species hybrid approaches that this experiment had set in train, and review their results from the standpoint of current insights. The history of Boveri's hybrid merogone experiment suggests important lessons about the interplay between what we call "models", the specific intellectual statements we conceive about how biology works, and the sometimes difficult task of generating experimental proof for these concepts.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalDevelopmental Biology
Volume314
Issue number1
DOIs
StatePublished - Feb 1 2008

Fingerprint

Sea Urchins
Chromosomes
History
Causality
Eggs
Embryonic Development
Spermatozoa
Research Design
Embryonic Structures

Keywords

  • Genomic control
  • Hybrid merogones
  • Sea urchin embryos
  • Species hybrids

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Boveri's long experiment : Sea urchin merogones and the establishment of the role of nuclear chromosomes in development. / Laubichler, Manfred; Davidson, Eric H.

In: Developmental Biology, Vol. 314, No. 1, 01.02.2008, p. 1-11.

Research output: Contribution to journalArticle

@article{3212420395c94f1bbdbb83dba7d8496b,
title = "Boveri's long experiment: Sea urchin merogones and the establishment of the role of nuclear chromosomes in development",
abstract = "Theodor Boveri's major intellectual contribution was his focus on the causality of nuclear chromosomal determinants for embryological development. His initial experimental attempt to demonstrate that the character of the developing embryo is determined by nuclear rather than cytoplasmic factors was launched in 1889. The experimental design was to fertilize enucleate sea urchin eggs with sperm of another species that produces a distinguishably different embryonic morphology. Boveri's {"}hybrid merogone{"} experiment provided what he initially thought was empirical evidence for the nuclear control of development. However, for subtle reasons, the data were not interpretable and the experiment was repeated and contested. At the end of his life, Boveri was finally able to explain the technical difficulties that had beset the original experiment. However, by 1902 Boveri had carried out his famous polyspermy experiments, which provided decisive evidence for the role of nuclear chromosomal determinants in embryogenesis. Here we present the history of the hybrid merogone experiment as an important case of conceptual reasoning paired with (often difficult) experimental approaches. We then trace the further history of the merogone and normal species hybrid approaches that this experiment had set in train, and review their results from the standpoint of current insights. The history of Boveri's hybrid merogone experiment suggests important lessons about the interplay between what we call {"}models{"}, the specific intellectual statements we conceive about how biology works, and the sometimes difficult task of generating experimental proof for these concepts.",
keywords = "Genomic control, Hybrid merogones, Sea urchin embryos, Species hybrids",
author = "Manfred Laubichler and Davidson, {Eric H.}",
year = "2008",
month = "2",
day = "1",
doi = "10.1016/j.ydbio.2007.11.024",
language = "English (US)",
volume = "314",
pages = "1--11",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Boveri's long experiment

T2 - Sea urchin merogones and the establishment of the role of nuclear chromosomes in development

AU - Laubichler, Manfred

AU - Davidson, Eric H.

PY - 2008/2/1

Y1 - 2008/2/1

N2 - Theodor Boveri's major intellectual contribution was his focus on the causality of nuclear chromosomal determinants for embryological development. His initial experimental attempt to demonstrate that the character of the developing embryo is determined by nuclear rather than cytoplasmic factors was launched in 1889. The experimental design was to fertilize enucleate sea urchin eggs with sperm of another species that produces a distinguishably different embryonic morphology. Boveri's "hybrid merogone" experiment provided what he initially thought was empirical evidence for the nuclear control of development. However, for subtle reasons, the data were not interpretable and the experiment was repeated and contested. At the end of his life, Boveri was finally able to explain the technical difficulties that had beset the original experiment. However, by 1902 Boveri had carried out his famous polyspermy experiments, which provided decisive evidence for the role of nuclear chromosomal determinants in embryogenesis. Here we present the history of the hybrid merogone experiment as an important case of conceptual reasoning paired with (often difficult) experimental approaches. We then trace the further history of the merogone and normal species hybrid approaches that this experiment had set in train, and review their results from the standpoint of current insights. The history of Boveri's hybrid merogone experiment suggests important lessons about the interplay between what we call "models", the specific intellectual statements we conceive about how biology works, and the sometimes difficult task of generating experimental proof for these concepts.

AB - Theodor Boveri's major intellectual contribution was his focus on the causality of nuclear chromosomal determinants for embryological development. His initial experimental attempt to demonstrate that the character of the developing embryo is determined by nuclear rather than cytoplasmic factors was launched in 1889. The experimental design was to fertilize enucleate sea urchin eggs with sperm of another species that produces a distinguishably different embryonic morphology. Boveri's "hybrid merogone" experiment provided what he initially thought was empirical evidence for the nuclear control of development. However, for subtle reasons, the data were not interpretable and the experiment was repeated and contested. At the end of his life, Boveri was finally able to explain the technical difficulties that had beset the original experiment. However, by 1902 Boveri had carried out his famous polyspermy experiments, which provided decisive evidence for the role of nuclear chromosomal determinants in embryogenesis. Here we present the history of the hybrid merogone experiment as an important case of conceptual reasoning paired with (often difficult) experimental approaches. We then trace the further history of the merogone and normal species hybrid approaches that this experiment had set in train, and review their results from the standpoint of current insights. The history of Boveri's hybrid merogone experiment suggests important lessons about the interplay between what we call "models", the specific intellectual statements we conceive about how biology works, and the sometimes difficult task of generating experimental proof for these concepts.

KW - Genomic control

KW - Hybrid merogones

KW - Sea urchin embryos

KW - Species hybrids

UR - http://www.scopus.com/inward/record.url?scp=38349190658&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38349190658&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2007.11.024

DO - 10.1016/j.ydbio.2007.11.024

M3 - Article

VL - 314

SP - 1

EP - 11

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -