Boosting d-lactate production in engineered cyanobacteria using sterilized anaerobic digestion effluents

Whitney D. Hollinshead; Arul M. Varman; Le You; Zachary Hembree; Yinjie J. Tang

doi:10.1016/j.biortech.2014.07.003

Boosting d-lactate production in engineered cyanobacteria using sterilized anaerobic digestion effluents

Whitney D. Hollinshead, Arul M. Varman, Le You, Zachary Hembree, Yinjie J. Tang

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Anaerobic digestion (AD) is an environmentally friendly approach to waste treatment, which can generate N and P-rich effluents that can be used as nutrient sources for microalgal cultivations. Modifications of AD processes to inhibit methanogenesis leads to the accumulation of acetic acid, a carbon source that can promote microalgal biosynthesis. This study tested different AD effluents from municipal wastes on their effect on d-lactate production by an engineered Synechocystis sp. PCC 6803 (carrying a novel lactate dehydrogenase). The results indicate that: (1) AD effluents can be supplemented into the modified BG-11 culture medium (up to 1:4 volume ratio) to reduce N and P cost; (2) acetate-rich AD effluents enhance d-lactate synthesis by ~40% (1.2. g/L of d-lactate in 20. days); and (3) neutral or acidic medium had a deleterious effect on lactate secretion and biomass growth by the engineered strain. This study demonstrates the advantages and guidelines in employing wastewater for photomixotrophic biosynthesis using engineered microalgae.

Original language	English (US)
Pages (from-to)	462-467
Number of pages	6
Journal	Bioresource Technology
Volume	169
DOIs	https://doi.org/10.1016/j.biortech.2014.07.003
State	Published - Oct 2014
Externally published	Yes

Keywords

D-lactate dehydrogenase
Municipal waste
Photomixotrophic
Synechocystis 6803

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

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10.1016/j.biortech.2014.07.003

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title = "Boosting d-lactate production in engineered cyanobacteria using sterilized anaerobic digestion effluents",

abstract = "Anaerobic digestion (AD) is an environmentally friendly approach to waste treatment, which can generate N and P-rich effluents that can be used as nutrient sources for microalgal cultivations. Modifications of AD processes to inhibit methanogenesis leads to the accumulation of acetic acid, a carbon source that can promote microalgal biosynthesis. This study tested different AD effluents from municipal wastes on their effect on d-lactate production by an engineered Synechocystis sp. PCC 6803 (carrying a novel lactate dehydrogenase). The results indicate that: (1) AD effluents can be supplemented into the modified BG-11 culture medium (up to 1:4 volume ratio) to reduce N and P cost; (2) acetate-rich AD effluents enhance d-lactate synthesis by ~40% (1.2. g/L of d-lactate in 20. days); and (3) neutral or acidic medium had a deleterious effect on lactate secretion and biomass growth by the engineered strain. This study demonstrates the advantages and guidelines in employing wastewater for photomixotrophic biosynthesis using engineered microalgae.",

keywords = "D-lactate dehydrogenase, Municipal waste, Photomixotrophic, Synechocystis 6803",

author = "Hollinshead, {Whitney D.} and Varman, {Arul M.} and Le You and Zachary Hembree and Tang, {Yinjie J.}",

note = "Funding Information: W.H., A.M.V. and Y.J.T. designed this research. W.H., A.M.V., L.Y. and Z.H. performed the experiments. W.H., A.M.V. and Y.J.T. wrote the paper. Every author revised the manuscript. We are thankful to Professor Yan Liu{\textquoteright}s group at Michigan State University for providing us with anaerobic digestion effluents. We also thank Katrina Leyden for her help in editing the paper. This work was supported by funding from the National Science Foundation ( MCB0954016 ).",

year = "2014",

month = oct,

doi = "10.1016/j.biortech.2014.07.003",

language = "English (US)",

volume = "169",

pages = "462--467",

journal = "Bioresource Technology",

issn = "0960-8524",

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T1 - Boosting d-lactate production in engineered cyanobacteria using sterilized anaerobic digestion effluents

AU - Hollinshead, Whitney D.

AU - Varman, Arul M.

AU - You, Le

AU - Hembree, Zachary

AU - Tang, Yinjie J.

N1 - Funding Information: W.H., A.M.V. and Y.J.T. designed this research. W.H., A.M.V., L.Y. and Z.H. performed the experiments. W.H., A.M.V. and Y.J.T. wrote the paper. Every author revised the manuscript. We are thankful to Professor Yan Liu’s group at Michigan State University for providing us with anaerobic digestion effluents. We also thank Katrina Leyden for her help in editing the paper. This work was supported by funding from the National Science Foundation ( MCB0954016 ).

PY - 2014/10

Y1 - 2014/10

N2 - Anaerobic digestion (AD) is an environmentally friendly approach to waste treatment, which can generate N and P-rich effluents that can be used as nutrient sources for microalgal cultivations. Modifications of AD processes to inhibit methanogenesis leads to the accumulation of acetic acid, a carbon source that can promote microalgal biosynthesis. This study tested different AD effluents from municipal wastes on their effect on d-lactate production by an engineered Synechocystis sp. PCC 6803 (carrying a novel lactate dehydrogenase). The results indicate that: (1) AD effluents can be supplemented into the modified BG-11 culture medium (up to 1:4 volume ratio) to reduce N and P cost; (2) acetate-rich AD effluents enhance d-lactate synthesis by ~40% (1.2. g/L of d-lactate in 20. days); and (3) neutral or acidic medium had a deleterious effect on lactate secretion and biomass growth by the engineered strain. This study demonstrates the advantages and guidelines in employing wastewater for photomixotrophic biosynthesis using engineered microalgae.

AB - Anaerobic digestion (AD) is an environmentally friendly approach to waste treatment, which can generate N and P-rich effluents that can be used as nutrient sources for microalgal cultivations. Modifications of AD processes to inhibit methanogenesis leads to the accumulation of acetic acid, a carbon source that can promote microalgal biosynthesis. This study tested different AD effluents from municipal wastes on their effect on d-lactate production by an engineered Synechocystis sp. PCC 6803 (carrying a novel lactate dehydrogenase). The results indicate that: (1) AD effluents can be supplemented into the modified BG-11 culture medium (up to 1:4 volume ratio) to reduce N and P cost; (2) acetate-rich AD effluents enhance d-lactate synthesis by ~40% (1.2. g/L of d-lactate in 20. days); and (3) neutral or acidic medium had a deleterious effect on lactate secretion and biomass growth by the engineered strain. This study demonstrates the advantages and guidelines in employing wastewater for photomixotrophic biosynthesis using engineered microalgae.

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KW - Municipal waste

KW - Photomixotrophic

KW - Synechocystis 6803

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Boosting d-lactate production in engineered cyanobacteria using sterilized anaerobic digestion effluents

Abstract

Keywords

ASJC Scopus subject areas

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