Abstract
Background: Aedes aegypti is a prominent and highly competent vector of several arboviral diseases, including dengue, yellow fever, and Zika. Behaviors associated with reproductive feeding, both pre-and post-blood meal, directly influence disease transmission capacity. Odors mediate host seeking pre-blood meal, while post-blood meal females are refractory to host odors for at least 24 h. During this time, flight activity is substantially reduced. Two key host odors, carbon dioxide and (R)-1-octen-3-ol, are detected by the maxillary palps in mosquitoes. In the search for future vector control tools, the identification of genes that are regulated in the maxillary palps between host seeking and 24 h post-blood meal may provide an informative pool of targets. Results: The blood meal-induced regulation of chemosensory, neuromodulatory, and other signal transduction genes was investigated in the maxillary palps of 24 h post-blood fed Ae. aegypti females, 6 days after emergence, and compared to host-seeking females of the same age using a transcriptomic approach. Genes-of-interest implicated in the behavioral switch from host seeking to post-blood meal quiescence were identified from multiple gene families investigated: odorant receptors, ionotropic receptors, pickpocket receptors, transient receptor potential receptors, odorant binding proteins, chemosensory proteins, neuromodulators, and their receptors, as well as constituents of second messenger signaling pathways. Reflecting the change in transcript abundance of families involved in CO2 signaling, the neural sensitivity to this key kairomone compound was found to decrease in blood fed mosquitoes compare with their on-blood fed counter parts. Conclusions: Sensory-associated gene expression is regulated in the maxillary palps of Ae. aegypti females in response to blood feeding. The concerted regulation of multiple genes within the sensory pathways of the maxillary palps likely play a key role in modulating the behavioral changes observed post-blood meal. Future functional characterization of the proteins generated by the genes-of-interest identified in this study may provide both a better understanding of the regulation of gonotrophic feeding and a pool of potential targets for vector control strategies.
| Original language | English (US) |
|---|---|
| Article number | 336 |
| Journal | Frontiers in Ecology and Evolution |
| Volume | 7 |
| Issue number | SEP |
| DOIs | |
| State | Published - Jan 1 2019 |
| Externally published | Yes |
Fingerprint
Keywords
- Blood feeding
- Chemosensory
- Host seeking
- Mosquito
- Resting
- Transcriptome
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Ecology
Cite this
Blood meal induced regulation of gene expression in the maxillary palps, a chemosensory organ of the mosquito Aedes aegypti. / Hill, Sharon R.; Ghaninia, Majid; Ignell, Rickard.
In: Frontiers in Ecology and Evolution, Vol. 7, No. SEP, 336, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Blood meal induced regulation of gene expression in the maxillary palps, a chemosensory organ of the mosquito Aedes aegypti
AU - Hill, Sharon R.
AU - Ghaninia, Majid
AU - Ignell, Rickard
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Background: Aedes aegypti is a prominent and highly competent vector of several arboviral diseases, including dengue, yellow fever, and Zika. Behaviors associated with reproductive feeding, both pre-and post-blood meal, directly influence disease transmission capacity. Odors mediate host seeking pre-blood meal, while post-blood meal females are refractory to host odors for at least 24 h. During this time, flight activity is substantially reduced. Two key host odors, carbon dioxide and (R)-1-octen-3-ol, are detected by the maxillary palps in mosquitoes. In the search for future vector control tools, the identification of genes that are regulated in the maxillary palps between host seeking and 24 h post-blood meal may provide an informative pool of targets. Results: The blood meal-induced regulation of chemosensory, neuromodulatory, and other signal transduction genes was investigated in the maxillary palps of 24 h post-blood fed Ae. aegypti females, 6 days after emergence, and compared to host-seeking females of the same age using a transcriptomic approach. Genes-of-interest implicated in the behavioral switch from host seeking to post-blood meal quiescence were identified from multiple gene families investigated: odorant receptors, ionotropic receptors, pickpocket receptors, transient receptor potential receptors, odorant binding proteins, chemosensory proteins, neuromodulators, and their receptors, as well as constituents of second messenger signaling pathways. Reflecting the change in transcript abundance of families involved in CO2 signaling, the neural sensitivity to this key kairomone compound was found to decrease in blood fed mosquitoes compare with their on-blood fed counter parts. Conclusions: Sensory-associated gene expression is regulated in the maxillary palps of Ae. aegypti females in response to blood feeding. The concerted regulation of multiple genes within the sensory pathways of the maxillary palps likely play a key role in modulating the behavioral changes observed post-blood meal. Future functional characterization of the proteins generated by the genes-of-interest identified in this study may provide both a better understanding of the regulation of gonotrophic feeding and a pool of potential targets for vector control strategies.
AB - Background: Aedes aegypti is a prominent and highly competent vector of several arboviral diseases, including dengue, yellow fever, and Zika. Behaviors associated with reproductive feeding, both pre-and post-blood meal, directly influence disease transmission capacity. Odors mediate host seeking pre-blood meal, while post-blood meal females are refractory to host odors for at least 24 h. During this time, flight activity is substantially reduced. Two key host odors, carbon dioxide and (R)-1-octen-3-ol, are detected by the maxillary palps in mosquitoes. In the search for future vector control tools, the identification of genes that are regulated in the maxillary palps between host seeking and 24 h post-blood meal may provide an informative pool of targets. Results: The blood meal-induced regulation of chemosensory, neuromodulatory, and other signal transduction genes was investigated in the maxillary palps of 24 h post-blood fed Ae. aegypti females, 6 days after emergence, and compared to host-seeking females of the same age using a transcriptomic approach. Genes-of-interest implicated in the behavioral switch from host seeking to post-blood meal quiescence were identified from multiple gene families investigated: odorant receptors, ionotropic receptors, pickpocket receptors, transient receptor potential receptors, odorant binding proteins, chemosensory proteins, neuromodulators, and their receptors, as well as constituents of second messenger signaling pathways. Reflecting the change in transcript abundance of families involved in CO2 signaling, the neural sensitivity to this key kairomone compound was found to decrease in blood fed mosquitoes compare with their on-blood fed counter parts. Conclusions: Sensory-associated gene expression is regulated in the maxillary palps of Ae. aegypti females in response to blood feeding. The concerted regulation of multiple genes within the sensory pathways of the maxillary palps likely play a key role in modulating the behavioral changes observed post-blood meal. Future functional characterization of the proteins generated by the genes-of-interest identified in this study may provide both a better understanding of the regulation of gonotrophic feeding and a pool of potential targets for vector control strategies.
KW - Blood feeding
KW - Chemosensory
KW - Host seeking
KW - Mosquito
KW - Resting
KW - Transcriptome
UR - http://www.scopus.com/inward/record.url?scp=85072914721&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85072914721&partnerID=8YFLogxK
U2 - 10.3389/fevo.2019.00336
DO - 10.3389/fevo.2019.00336
M3 - Article
AN - SCOPUS:85072914721
VL - 7
JO - Frontiers in Ecology and Evolution
JF - Frontiers in Ecology and Evolution
SN - 2296-701X
IS - SEP
M1 - 336
ER -