Blockade of interferon induction and action by the E3L double-stranded RNA binding proteins of vaccinia virus

Ying Xiang, Richard C. Condit, Sangeetha Vijaysri, Bertram Jacobs, Bryan R G Williams, Robert H. Silverman

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

The vaccinia virus E3L gene encodes two double-stranded RNA binding proteins that promote viral growth and pathogenesis through suppression of innate immunity. To explore how E3L enables vaccinia virus to evade the interferon system, cells and mice deficient in the principal interferon-regulated antiviral enzymes, PKR and RNase L, were infected with wild-type vaccinia virus and strains of vaccinia virus from which E3L had been deleted (E3L-deleted strains). While wild-type virus was unaffected by RNase L and PKR, virus lacking E3L replicated only in the deficient cells. Nevertheless, E3L-deleted virus failed to replicate to high titers or to cause significant morbidity or mortality in triply deficient mice lacking RNase L, PKR, and Mx1. To investigate the underlying cause, we determined the effect of E3L on interferon regulatory factor 3 (IRF3), a transcription factor required for viral induction of subtypes of type I interferons. Results showed that IRF3 activation and interferon-β induction occurred after infections with E3L-deleted virus but not with wild-type virus. These findings demonstrate that E3L plays an essential role in the pathogenesis of vaccinia virus by blocking the interferon system at multiple levels. Furthermore, our results indicate the existence of an interferon-mediated antipoxvirus pathway that operates independently of PKR, Mx1, or the 2-5A/RNase L system.

Original languageEnglish (US)
Pages (from-to)5251-5259
Number of pages9
JournalJournal of Virology
Volume76
Issue number10
DOIs
StatePublished - 2002
Externally publishedYes

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Vaccinia virus
RNA-binding proteins
RNA-Binding Proteins
double-stranded RNA
interferons
Interferons
Viruses
Interferon Regulatory Factor-3
viruses
pathogenesis
Interferon Type I
Innate Immunity
mice
Antiviral Agents
Transcription Factors
morbidity
microbial growth
Morbidity
transcription factors
cells

ASJC Scopus subject areas

  • Immunology

Cite this

Blockade of interferon induction and action by the E3L double-stranded RNA binding proteins of vaccinia virus. / Xiang, Ying; Condit, Richard C.; Vijaysri, Sangeetha; Jacobs, Bertram; Williams, Bryan R G; Silverman, Robert H.

In: Journal of Virology, Vol. 76, No. 10, 2002, p. 5251-5259.

Research output: Contribution to journalArticle

Xiang, Ying ; Condit, Richard C. ; Vijaysri, Sangeetha ; Jacobs, Bertram ; Williams, Bryan R G ; Silverman, Robert H. / Blockade of interferon induction and action by the E3L double-stranded RNA binding proteins of vaccinia virus. In: Journal of Virology. 2002 ; Vol. 76, No. 10. pp. 5251-5259.
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