Biscoumarin derivatives from Edgeworthia gardneri that inhibit the lyase activity of DNA polymerase β

Shi Sheng Li, Zhijie Gao, Xizhi Feng, Sidney Hecht

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Bioassay-guided fractionation of an active methyl ethyl ketone extract of Edgeworthia gardneri, using an assay to monitor DNA polymerase β lyase inhibition, resulted in the isolation of three known biscoumarin derivatives, 7-hydroxy-3,7′-dicoumaryl ether (edgeworin, 1), 7-hydroxy-6-methoxy-3, 7′-dicoumaryl ether (daphnoretin, 2), and 6,7-dihydroxy-3,7′- dicoumaryl ether (edgeworthin, 3). Compounds 1-3 inhibited the lyase activity of DNA polymerase β with IC50 values of 7.3 μg/mL (22.5 μM), 43.0 μg/mL (122.3 μM), and 32.1 μg/mL (94.8 μM), respectively.

Original languageEnglish (US)
Pages (from-to)1608-1610
Number of pages3
JournalJournal of Natural Products
Volume67
Issue number9
DOIs
StatePublished - Sep 2004
Externally publishedYes

Fingerprint

Edgeworthia
Lyases
lyases
DNA-directed DNA polymerase
DNA-Directed DNA Polymerase
Ether
ethers
chemical derivatives
Derivatives
Bioassay
Fractionation
ketones
Biological Assay
Inhibitory Concentration 50
inhibitory concentration 50
Assays
fractionation
bioassays
extracts
assays

ASJC Scopus subject areas

  • Plant Science
  • Chemistry (miscellaneous)
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Biscoumarin derivatives from Edgeworthia gardneri that inhibit the lyase activity of DNA polymerase β. / Li, Shi Sheng; Gao, Zhijie; Feng, Xizhi; Hecht, Sidney.

In: Journal of Natural Products, Vol. 67, No. 9, 09.2004, p. 1608-1610.

Research output: Contribution to journalArticle

@article{c49f8501d2624ab6a2778d11f40667aa,
title = "Biscoumarin derivatives from Edgeworthia gardneri that inhibit the lyase activity of DNA polymerase β",
abstract = "Bioassay-guided fractionation of an active methyl ethyl ketone extract of Edgeworthia gardneri, using an assay to monitor DNA polymerase β lyase inhibition, resulted in the isolation of three known biscoumarin derivatives, 7-hydroxy-3,7′-dicoumaryl ether (edgeworin, 1), 7-hydroxy-6-methoxy-3, 7′-dicoumaryl ether (daphnoretin, 2), and 6,7-dihydroxy-3,7′- dicoumaryl ether (edgeworthin, 3). Compounds 1-3 inhibited the lyase activity of DNA polymerase β with IC50 values of 7.3 μg/mL (22.5 μM), 43.0 μg/mL (122.3 μM), and 32.1 μg/mL (94.8 μM), respectively.",
author = "Li, {Shi Sheng} and Zhijie Gao and Xizhi Feng and Sidney Hecht",
year = "2004",
month = "9",
doi = "10.1021/np040127s",
language = "English (US)",
volume = "67",
pages = "1608--1610",
journal = "Journal of Natural Products",
issn = "0163-3864",
publisher = "American Chemical Society",
number = "9",

}

TY - JOUR

T1 - Biscoumarin derivatives from Edgeworthia gardneri that inhibit the lyase activity of DNA polymerase β

AU - Li, Shi Sheng

AU - Gao, Zhijie

AU - Feng, Xizhi

AU - Hecht, Sidney

PY - 2004/9

Y1 - 2004/9

N2 - Bioassay-guided fractionation of an active methyl ethyl ketone extract of Edgeworthia gardneri, using an assay to monitor DNA polymerase β lyase inhibition, resulted in the isolation of three known biscoumarin derivatives, 7-hydroxy-3,7′-dicoumaryl ether (edgeworin, 1), 7-hydroxy-6-methoxy-3, 7′-dicoumaryl ether (daphnoretin, 2), and 6,7-dihydroxy-3,7′- dicoumaryl ether (edgeworthin, 3). Compounds 1-3 inhibited the lyase activity of DNA polymerase β with IC50 values of 7.3 μg/mL (22.5 μM), 43.0 μg/mL (122.3 μM), and 32.1 μg/mL (94.8 μM), respectively.

AB - Bioassay-guided fractionation of an active methyl ethyl ketone extract of Edgeworthia gardneri, using an assay to monitor DNA polymerase β lyase inhibition, resulted in the isolation of three known biscoumarin derivatives, 7-hydroxy-3,7′-dicoumaryl ether (edgeworin, 1), 7-hydroxy-6-methoxy-3, 7′-dicoumaryl ether (daphnoretin, 2), and 6,7-dihydroxy-3,7′- dicoumaryl ether (edgeworthin, 3). Compounds 1-3 inhibited the lyase activity of DNA polymerase β with IC50 values of 7.3 μg/mL (22.5 μM), 43.0 μg/mL (122.3 μM), and 32.1 μg/mL (94.8 μM), respectively.

UR - http://www.scopus.com/inward/record.url?scp=4644233473&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4644233473&partnerID=8YFLogxK

U2 - 10.1021/np040127s

DO - 10.1021/np040127s

M3 - Article

C2 - 15387673

AN - SCOPUS:4644233473

VL - 67

SP - 1608

EP - 1610

JO - Journal of Natural Products

JF - Journal of Natural Products

SN - 0163-3864

IS - 9

ER -