TY - JOUR
T1 - Biotechnological and protein-engineering implications of ancestral protein resurrection
AU - Risso, Valeria A.
AU - Sanchez-Ruiz, Jose M.
AU - Ozkan, Sefika
N1 - Funding Information:
VAR and JMS-R are supported by Feder Funds, Grant BIO2015-66426-R from the Spanish Ministry of Economy and Competitiveness and Grant RGP0041/2017 from the Human Frontier Science Program. Support from NSF-MCB Award 1715591 and Scialog Fellow Award by RCS and the Gordon & Betty Moore Foundation is gratefully acknowledged by SBO.
PY - 2018/8
Y1 - 2018/8
N2 - Approximations to the sequences of ancestral proteins can be derived from the sequences of their modern descendants. Proteins encoded by such reconstructed sequences can be prepared in the laboratory and subjected to experimental scrutiny. These ‘resurrected’ ancestral proteins often display remarkable properties, reflecting ancestral adaptations to intra-cellular and extra-cellular environments that differed from the environments hosting modern/extant proteins. Recent experimental and computational work has specifically discussed high stability, substrate and catalytic promiscuity, conformational flexibility/diversity and altered patterns of interaction with other sub-cellular components. In this review, we discuss these remarkable properties as well as recent attempts to explore their biotechnological and protein-engineering potential.
AB - Approximations to the sequences of ancestral proteins can be derived from the sequences of their modern descendants. Proteins encoded by such reconstructed sequences can be prepared in the laboratory and subjected to experimental scrutiny. These ‘resurrected’ ancestral proteins often display remarkable properties, reflecting ancestral adaptations to intra-cellular and extra-cellular environments that differed from the environments hosting modern/extant proteins. Recent experimental and computational work has specifically discussed high stability, substrate and catalytic promiscuity, conformational flexibility/diversity and altered patterns of interaction with other sub-cellular components. In this review, we discuss these remarkable properties as well as recent attempts to explore their biotechnological and protein-engineering potential.
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U2 - 10.1016/j.sbi.2018.02.007
DO - 10.1016/j.sbi.2018.02.007
M3 - Review article
C2 - 29660672
AN - SCOPUS:85045643656
SN - 0959-440X
VL - 51
SP - 106
EP - 115
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
ER -