Abstract
The structural and functional properties of the reovirus serotype 1 (Lang strain) sl mRNA were examined. Reovirus s-class mRNAs, synthesized either in vivo within infected mouse L cells or in vitro by chymotrypsin-derived cores of purified virions, were purified by filter-hybridization using cDNA clones of the S-class genome segments. S1 cDNA-selected mRNA encoded the synthesis of the Mr ∼ 12,000 nonstructural polypeptide designated σ1bNS in addition to the well-established structural polypeptide σ1 now designated σ1a. The coding properties of in vivo- and in vitro-synthesized s1 mRNA were equivalent: both encoded σ1a and σ1bNS. Primer extension analysis of s1 mRNA revealed a single major 5′ terminus for both in vivo- and in vitro-synthesized s1 mRNA. These results suggest that there is a single transcript of the reovirus S1 genome segment which is functionally dicistronic, and likely encodes both σ1a and σ1bNS.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 9-18 |
| Number of pages | 10 |
| Journal | Virology |
| Volume | 147 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1985 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Biosynthesis of reovirus-specified polypeptides The s1 mRNA synthesized in vivo is structurally and functionally indistinguishable from in vitro-synthesized s1 mRNA and encodes two polypeptides, σ1a and σ1bNS . / Jacobs, Bertram; Atwater, Jonathan A.; Munemitsu, Susan M.; Samuel, Charles E.
In: Virology, Vol. 147, No. 1, 1985, p. 9-18.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biosynthesis of reovirus-specified polypeptides The s1 mRNA synthesized in vivo is structurally and functionally indistinguishable from in vitro-synthesized s1 mRNA and encodes two polypeptides, σ1a and σ1bNS
AU - Jacobs, Bertram
AU - Atwater, Jonathan A.
AU - Munemitsu, Susan M.
AU - Samuel, Charles E.
PY - 1985
Y1 - 1985
N2 - The structural and functional properties of the reovirus serotype 1 (Lang strain) sl mRNA were examined. Reovirus s-class mRNAs, synthesized either in vivo within infected mouse L cells or in vitro by chymotrypsin-derived cores of purified virions, were purified by filter-hybridization using cDNA clones of the S-class genome segments. S1 cDNA-selected mRNA encoded the synthesis of the Mr ∼ 12,000 nonstructural polypeptide designated σ1bNS in addition to the well-established structural polypeptide σ1 now designated σ1a. The coding properties of in vivo- and in vitro-synthesized s1 mRNA were equivalent: both encoded σ1a and σ1bNS. Primer extension analysis of s1 mRNA revealed a single major 5′ terminus for both in vivo- and in vitro-synthesized s1 mRNA. These results suggest that there is a single transcript of the reovirus S1 genome segment which is functionally dicistronic, and likely encodes both σ1a and σ1bNS.
AB - The structural and functional properties of the reovirus serotype 1 (Lang strain) sl mRNA were examined. Reovirus s-class mRNAs, synthesized either in vivo within infected mouse L cells or in vitro by chymotrypsin-derived cores of purified virions, were purified by filter-hybridization using cDNA clones of the S-class genome segments. S1 cDNA-selected mRNA encoded the synthesis of the Mr ∼ 12,000 nonstructural polypeptide designated σ1bNS in addition to the well-established structural polypeptide σ1 now designated σ1a. The coding properties of in vivo- and in vitro-synthesized s1 mRNA were equivalent: both encoded σ1a and σ1bNS. Primer extension analysis of s1 mRNA revealed a single major 5′ terminus for both in vivo- and in vitro-synthesized s1 mRNA. These results suggest that there is a single transcript of the reovirus S1 genome segment which is functionally dicistronic, and likely encodes both σ1a and σ1bNS.
UR - http://www.scopus.com/inward/record.url?scp=0022342818&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022342818&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(85)90222-3
DO - 10.1016/0042-6822(85)90222-3
M3 - Article
C2 - 2998074
AN - SCOPUS:0022342818
VL - 147
SP - 9
EP - 18
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -