Biosynthesis of reovirus-specified polypeptides The s1 mRNA synthesized in vivo is structurally and functionally indistinguishable from in vitro-synthesized s1 mRNA and encodes two polypeptides, σ1a and σ1bNS

Bertram Jacobs, Jonathan A. Atwater, Susan M. Munemitsu, Charles E. Samuel

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The structural and functional properties of the reovirus serotype 1 (Lang strain) sl mRNA were examined. Reovirus s-class mRNAs, synthesized either in vivo within infected mouse L cells or in vitro by chymotrypsin-derived cores of purified virions, were purified by filter-hybridization using cDNA clones of the S-class genome segments. S1 cDNA-selected mRNA encoded the synthesis of the Mr ∼ 12,000 nonstructural polypeptide designated σ1bNS in addition to the well-established structural polypeptide σ1 now designated σ1a. The coding properties of in vivo- and in vitro-synthesized s1 mRNA were equivalent: both encoded σ1a and σ1bNS. Primer extension analysis of s1 mRNA revealed a single major 5′ terminus for both in vivo- and in vitro-synthesized s1 mRNA. These results suggest that there is a single transcript of the reovirus S1 genome segment which is functionally dicistronic, and likely encodes both σ1a and σ1bNS.

Original languageEnglish (US)
Pages (from-to)9-18
Number of pages10
JournalVirology
Volume147
Issue number1
DOIs
StatePublished - 1985
Externally publishedYes

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Messenger RNA
Peptides
Mammalian Orthoreovirus
Complementary DNA
Genome
Chymotrypsin
Virion
In Vitro Techniques
Clone Cells

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

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title = "Biosynthesis of reovirus-specified polypeptides The s1 mRNA synthesized in vivo is structurally and functionally indistinguishable from in vitro-synthesized s1 mRNA and encodes two polypeptides, σ1a and σ1bNS",
abstract = "The structural and functional properties of the reovirus serotype 1 (Lang strain) sl mRNA were examined. Reovirus s-class mRNAs, synthesized either in vivo within infected mouse L cells or in vitro by chymotrypsin-derived cores of purified virions, were purified by filter-hybridization using cDNA clones of the S-class genome segments. S1 cDNA-selected mRNA encoded the synthesis of the Mr ∼ 12,000 nonstructural polypeptide designated σ1bNS in addition to the well-established structural polypeptide σ1 now designated σ1a. The coding properties of in vivo- and in vitro-synthesized s1 mRNA were equivalent: both encoded σ1a and σ1bNS. Primer extension analysis of s1 mRNA revealed a single major 5′ terminus for both in vivo- and in vitro-synthesized s1 mRNA. These results suggest that there is a single transcript of the reovirus S1 genome segment which is functionally dicistronic, and likely encodes both σ1a and σ1bNS.",
author = "Bertram Jacobs and Atwater, {Jonathan A.} and Munemitsu, {Susan M.} and Samuel, {Charles E.}",
year = "1985",
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TY - JOUR

T1 - Biosynthesis of reovirus-specified polypeptides The s1 mRNA synthesized in vivo is structurally and functionally indistinguishable from in vitro-synthesized s1 mRNA and encodes two polypeptides, σ1a and σ1bNS

AU - Jacobs, Bertram

AU - Atwater, Jonathan A.

AU - Munemitsu, Susan M.

AU - Samuel, Charles E.

PY - 1985

Y1 - 1985

N2 - The structural and functional properties of the reovirus serotype 1 (Lang strain) sl mRNA were examined. Reovirus s-class mRNAs, synthesized either in vivo within infected mouse L cells or in vitro by chymotrypsin-derived cores of purified virions, were purified by filter-hybridization using cDNA clones of the S-class genome segments. S1 cDNA-selected mRNA encoded the synthesis of the Mr ∼ 12,000 nonstructural polypeptide designated σ1bNS in addition to the well-established structural polypeptide σ1 now designated σ1a. The coding properties of in vivo- and in vitro-synthesized s1 mRNA were equivalent: both encoded σ1a and σ1bNS. Primer extension analysis of s1 mRNA revealed a single major 5′ terminus for both in vivo- and in vitro-synthesized s1 mRNA. These results suggest that there is a single transcript of the reovirus S1 genome segment which is functionally dicistronic, and likely encodes both σ1a and σ1bNS.

AB - The structural and functional properties of the reovirus serotype 1 (Lang strain) sl mRNA were examined. Reovirus s-class mRNAs, synthesized either in vivo within infected mouse L cells or in vitro by chymotrypsin-derived cores of purified virions, were purified by filter-hybridization using cDNA clones of the S-class genome segments. S1 cDNA-selected mRNA encoded the synthesis of the Mr ∼ 12,000 nonstructural polypeptide designated σ1bNS in addition to the well-established structural polypeptide σ1 now designated σ1a. The coding properties of in vivo- and in vitro-synthesized s1 mRNA were equivalent: both encoded σ1a and σ1bNS. Primer extension analysis of s1 mRNA revealed a single major 5′ terminus for both in vivo- and in vitro-synthesized s1 mRNA. These results suggest that there is a single transcript of the reovirus S1 genome segment which is functionally dicistronic, and likely encodes both σ1a and σ1bNS.

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