TY - JOUR
T1 - Biosensor analysis of natural and artificial sweeteners in intact taste epithelium
AU - Zhang, Fenni
AU - Zhang, Qian
AU - Zhang, Diming
AU - Lu, Yanli
AU - Liu, Qingjun
AU - Wang, Ping
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant nos. 81371643 and 61320106002 ) and the Zhejiang Provincial Natural Science Foundation of China (Grant no. LR13H180002 ).
PY - 2014/4/15
Y1 - 2014/4/15
N2 - Sweeteners are commonly used as food additives in our daily life, which, however, have been causing a number of undesirable diseases since the last century. Therefore, the detection and quantification of sweeteners are of great value for food safety. In this study, we used a taste biosensor to measure and analyze different sweeteners, both natural and artificial sweeteners included. Electrophysiological activities from taste epithelium were detected by the multi-channel biosensors and analyzed with spatiotemporal methods. The longtime signal result showed different temporal-frequency properties with stimulations of individual sweeteners such as glucose, sucrose, saccharin, and cyclamate, while the multi-channel results in our study revealed the spatial expression of taste epithelium to sweet stimuli. Furthermore, in the analysis of sweetener with different concentrations, the result showed obvious dose-dependent increases in signal responses of the taste epithelium, which indicated promising applications in sweetness evaluation. Besides, the mixture experiment of two natural sweeteners with a similar functional unit (glucose and sucrose) presented two signal patterns, which turned out to be similar with responses of each individual stimulus involved. The biosensor analysis of common sweeteners provided new approaches for both natural and artificial sweeteners evaluation.
AB - Sweeteners are commonly used as food additives in our daily life, which, however, have been causing a number of undesirable diseases since the last century. Therefore, the detection and quantification of sweeteners are of great value for food safety. In this study, we used a taste biosensor to measure and analyze different sweeteners, both natural and artificial sweeteners included. Electrophysiological activities from taste epithelium were detected by the multi-channel biosensors and analyzed with spatiotemporal methods. The longtime signal result showed different temporal-frequency properties with stimulations of individual sweeteners such as glucose, sucrose, saccharin, and cyclamate, while the multi-channel results in our study revealed the spatial expression of taste epithelium to sweet stimuli. Furthermore, in the analysis of sweetener with different concentrations, the result showed obvious dose-dependent increases in signal responses of the taste epithelium, which indicated promising applications in sweetness evaluation. Besides, the mixture experiment of two natural sweeteners with a similar functional unit (glucose and sucrose) presented two signal patterns, which turned out to be similar with responses of each individual stimulus involved. The biosensor analysis of common sweeteners provided new approaches for both natural and artificial sweeteners evaluation.
KW - Artificial sweetener
KW - Biosensor
KW - Microelectrodes
KW - Natural sugar
KW - Taste epithelium
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U2 - 10.1016/j.bios.2013.11.020
DO - 10.1016/j.bios.2013.11.020
M3 - Article
C2 - 24292144
AN - SCOPUS:84888414059
SN - 0956-5663
VL - 54
SP - 385
EP - 392
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -