TY - JOUR
T1 - Bioreduction of Antimonate by Anaerobic Methane Oxidation in a Membrane Biofilm Batch Reactor
AU - Lai, Chun Yu
AU - Dong, Qiu Yi
AU - Rittmann, Bruce
AU - Zhao, He Ping
N1 - Funding Information:
The authors thank the “National Key Technology R&D Program (2017ZX07206-002)”, the “National Natural Science Foundation of China (Grant No. 21577123)”, the “Natural Science Funds for Distinguished Young Scholar of Zhejiang Province (LR17B070001)”, and the “Fundamental Research Funds for the Central Universities (2016QNA6007, 2017XZZX010-03)” for their financial support.
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/8/7
Y1 - 2018/8/7
N2 - Employing a special anaerobic membrane biofilm batch reactor (MBBR), we demonstrated antimonate (Sb(V)) reduction using methane (CH4) as the sole electron donor. Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and Raman and photoluminescence (PL) spectra identified that Sb2O3 microcrystals were the main reduced products. The Sb(V) reduction rate increased continually over the 111-day experiment, which supports the enrichment of the microorganisms responsible for Sb(V) reduction to Sb(III). Copy numbers of the mcrA gene and archaeal and bacterial 16 S rRNA genes increased in parallel. Clone library and Illumina sequencing of 16S rRNA gene demonstrated that Methanosarcina became the dominant archaea in the biofilm, suggesting that Methanosarcina might play an important role in Sb(V) reduction in the CH4-based MBBR.
AB - Employing a special anaerobic membrane biofilm batch reactor (MBBR), we demonstrated antimonate (Sb(V)) reduction using methane (CH4) as the sole electron donor. Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and Raman and photoluminescence (PL) spectra identified that Sb2O3 microcrystals were the main reduced products. The Sb(V) reduction rate increased continually over the 111-day experiment, which supports the enrichment of the microorganisms responsible for Sb(V) reduction to Sb(III). Copy numbers of the mcrA gene and archaeal and bacterial 16 S rRNA genes increased in parallel. Clone library and Illumina sequencing of 16S rRNA gene demonstrated that Methanosarcina became the dominant archaea in the biofilm, suggesting that Methanosarcina might play an important role in Sb(V) reduction in the CH4-based MBBR.
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U2 - 10.1021/acs.est.8b02035
DO - 10.1021/acs.est.8b02035
M3 - Article
C2 - 30001126
AN - SCOPUS:85050029124
SN - 0013-936X
VL - 52
SP - 8693
EP - 8700
JO - Environmental Science and Technology
JF - Environmental Science and Technology
IS - 15
ER -