Bioprospecting of Native Efflux Pumps To Enhance Furfural Tolerance in Ethanologenic Escherichia coli

Gavin Kurgan, Larry A. Panyon, Yesenia Rodriguez-Sanchez, Eric Pacheco, Lizbeth M. Nieves, Robert Mann, David Nielsen, Xuan Wang

Research output: Contribution to journalArticle

Abstract

Efficient microbial conversion of lignocellulose into valuable products is often hindered by the presence of furfural, a dehydration product of pentoses in hemicellulose sugar syrups derived from woody biomass. For a cost-effective lignocellulose microbial conversion, robust biocatalysts are needed that can tolerate toxic inhibitors while maintaining optimal metabolic activities. A comprehensive plasmid-based library encoding native multidrug resistance (MDR) efflux pumps, porins, and select exporters from Escherichia coli was screened for furfural tolerance in an ethanologenic E. coli strain. Small multidrug resistance (SMR) pumps, such as SugE and MdtJI, as well as a lactate/glycolate:H+ symporter, LldP, conferred furfural tolerance in liquid culture tests. Expression of the SMR pump potentially increased furfural efflux and cellular viability upon furfural assault, suggesting novel activities for SMR pumps as furfural efflux proteins. Furthermore, induced expression of mdtJI enhanced ethanol fermentative production of LY180 in the presence of furfural or 5-hydroxymethylfurfural, further demonstrating the applications of SMR pumps. This work describes an effective approach to identify useful efflux systems with desired activities for nonnative toxic chemicals and provides a platform to further enhance furfural efflux by protein engineering and mutagenesis.IMPORTANCE Lignocellulosic biomass, especially agricultural residues, represents an important potential feedstock for microbial production of renewable fuels and chemicals. During the deconstruction of hemicellulose by thermochemical processes, side products that inhibit cell growth and production, such as furan aldehydes, are generated, limiting cost-effective lignocellulose conversion. Here, we developed a new approach to increase cellular tolerance by expressing multidrug resistance (MDR) pumps with putative efflux activities for furan aldehydes. The developed plasmid library and screening methods may facilitate new discoveries of MDR pumps for diverse toxic chemicals important for microbial conversion.

Original languageEnglish (US)
JournalApplied and environmental microbiology
Volume85
Issue number6
DOIs
StatePublished - Mar 15 2019

Fingerprint

Furaldehyde
furfural
multiple drug resistance
Multiple Drug Resistance
transporters
pump
tolerance
pumps
Escherichia coli
lignocellulose
Poisons
furan
aldehyde
glycolic acid
furans
plasmid
toxic substances
hemicellulose
Aldehydes
Biomass

Keywords

  • Escherichia coli
  • furfural
  • lignocellulose
  • metabolic engineering
  • multidrug resistance pumps

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Bioprospecting of Native Efflux Pumps To Enhance Furfural Tolerance in Ethanologenic Escherichia coli. / Kurgan, Gavin; Panyon, Larry A.; Rodriguez-Sanchez, Yesenia; Pacheco, Eric; Nieves, Lizbeth M.; Mann, Robert; Nielsen, David; Wang, Xuan.

In: Applied and environmental microbiology, Vol. 85, No. 6, 15.03.2019.

Research output: Contribution to journalArticle

Kurgan, Gavin ; Panyon, Larry A. ; Rodriguez-Sanchez, Yesenia ; Pacheco, Eric ; Nieves, Lizbeth M. ; Mann, Robert ; Nielsen, David ; Wang, Xuan. / Bioprospecting of Native Efflux Pumps To Enhance Furfural Tolerance in Ethanologenic Escherichia coli. In: Applied and environmental microbiology. 2019 ; Vol. 85, No. 6.
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