Biophysical separation of Staphylococcus epidermidis strains based on antibiotic resistance

Paul V. Jones, Shannon Huey, Paige Davis, Ryan Yanashima, Ryan McLemore, Alex McLaren, Mark Hayes

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Electrophoretic and dielectrophoretic approaches to separations can provide unique capabilities. In the past, capillary and microchip-based approaches to electrophoresis have demonstrated extremely high-resolution separations. More recently, dielectrophoretic systems have shown excellent results for the separation of bioparticles. Here we demonstrate resolution of a difficult pair of targets: gentamicin resistant and susceptible strains of Staphylococcus epidermidis. This separation has significant potential implications for healthcare. This establishes a foundation for biophysical separations as a direct diagnostic tool, potentially improving nearly every figure of merit for diagnostics and antibiotic stewardship. The separations are performed on a modified gradient insulator-based dielectrophoresis (g-iDEP) system and demonstrate that the presence of antibiotic resistance enzymes (or secondary effects) produces a sufficient degree of electrophysical difference to allow separation. The differentiating factor is the ratio of electrophoretic to dielectrophoretic mobilities. This factor is 4.6 ± 0.6 × 10(9) V m(-2) for the resistant strain, versus 9.2 ± 0.4 × 10(9) V m(-2) for the susceptible strain. Using g-iDEP separation, this difference produces clear and easily discerned differentiation of the two strains.

Original languageEnglish (US)
Pages (from-to)5152-5161
Number of pages10
JournalThe Analyst
Volume140
Issue number15
DOIs
StatePublished - Aug 7 2015

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Staphylococcus epidermidis
antibiotic resistance
Antibiotics
Microbial Drug Resistance
Gentamicins
Electrophoresis
Anti-Bacterial Agents
Delivery of Health Care
Enzymes
antibiotics
health care
electrokinesis
enzyme

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Biochemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Jones, P. V., Huey, S., Davis, P., Yanashima, R., McLemore, R., McLaren, A., & Hayes, M. (2015). Biophysical separation of Staphylococcus epidermidis strains based on antibiotic resistance. The Analyst, 140(15), 5152-5161. https://doi.org/10.1039/c5an00906e

Biophysical separation of Staphylococcus epidermidis strains based on antibiotic resistance. / Jones, Paul V.; Huey, Shannon; Davis, Paige; Yanashima, Ryan; McLemore, Ryan; McLaren, Alex; Hayes, Mark.

In: The Analyst, Vol. 140, No. 15, 07.08.2015, p. 5152-5161.

Research output: Contribution to journalArticle

Jones, PV, Huey, S, Davis, P, Yanashima, R, McLemore, R, McLaren, A & Hayes, M 2015, 'Biophysical separation of Staphylococcus epidermidis strains based on antibiotic resistance', The Analyst, vol. 140, no. 15, pp. 5152-5161. https://doi.org/10.1039/c5an00906e
Jones PV, Huey S, Davis P, Yanashima R, McLemore R, McLaren A et al. Biophysical separation of Staphylococcus epidermidis strains based on antibiotic resistance. The Analyst. 2015 Aug 7;140(15):5152-5161. https://doi.org/10.1039/c5an00906e
Jones, Paul V. ; Huey, Shannon ; Davis, Paige ; Yanashima, Ryan ; McLemore, Ryan ; McLaren, Alex ; Hayes, Mark. / Biophysical separation of Staphylococcus epidermidis strains based on antibiotic resistance. In: The Analyst. 2015 ; Vol. 140, No. 15. pp. 5152-5161.
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