Abstract
An automated biomechatronic small fluid sample preparation system for DNA processing has been developed in the Genomation Laboratory of the University of Washington Department of Electrical Engineering. The system automates steps necessary to prepare DNA for sequencing using sample volumes ten times smaller than the current state of the art manual and automated instrumentation. This will significantly reduce the time and cost of DNA sequencing and will help the Human Genome Project meet its goal of determining all three billion bases of human DNA sequence by the year 2005. The biomechatronic system comprising electromagnetic actuators, piezoceramic actuators, pneumatic pumps, linear mechanisms, thermal controllers, optical sensors, electronics, computer control and software is described in detail. Successful experimental results including restriction enzyme digests and polymerase chain reactions (PCRs) performed with this novel system are presented.
| Original language | English (US) |
|---|---|
| Title of host publication | IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM |
| Place of Publication | Piscataway, NJ, United States |
| Publisher | IEEE |
| Pages | 6 |
| Number of pages | 1 |
| State | Published - 1997 |
| Externally published | Yes |
| Event | Proceedings of the 1997 1st IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM'97 - Tokyo, Jpn Duration: Jun 16 1997 → Jun 20 1997 |
Other
| Other | Proceedings of the 1997 1st IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM'97 |
|---|---|
| City | Tokyo, Jpn |
| Period | 6/16/97 → 6/20/97 |
ASJC Scopus subject areas
- General Engineering