Biological force measurement in a protein-based nanoactuator

Gaurav Sharma, Kaushal Rege, David E. Budil, Martin Yarmush, Constantinos Mavroidis

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The mechanical force exerted by a nanoactuator due to pH actuation is discussed from a statistical mechanics and free energy of conformational change viewpoint. We use molecular dynamics to show that the nanoactuator, based on the coiled-coil leucine zipper portion of a yeast transcriptional activator protein, can generate mechanical forces of the order of 2040 pN upon pH modulation. The forces are generated due to the electrostatic repulsions at low pH between His-tag handles and other charged residues engineered into the protein sequence. The biological force output of the nanoactuator is comparable to that generated by adenosine triphosphate (ATP)-based molecular motors such as myosin and kinesin even though the nanoactuator is smaller in size to these molecular motors. The force calculation technique can readily be applied to other biomolecular systems and has implications in the area of bionanotechnology and in particular to study and characterize the properties of novel protein and DNA-based nanodevices.

Original languageEnglish (US)
Article number5062286
Pages (from-to)684-691
Number of pages8
JournalIEEE Transactions on Nanotechnology
Volume8
Issue number6
DOIs
StatePublished - Nov 1 2009

Keywords

  • Biomolecular devices
  • Coiled-coil protein
  • Force measurement
  • Nanotechnology

ASJC Scopus subject areas

  • Computer Science Applications
  • Electrical and Electronic Engineering

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