Bioinformatics and fluorescence dnaser for NAPPA studies on cell transformation and cell cycle

Victor Sivozhelezov, Rosanna Spera, Luca Giacomelli, Eugenie Hainsworth, Joshua LaBaer, Nicola N. Bragazzi, Claudio Nicolini

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations

Abstract

We present the results obtained analyzing for the first time a fluorescent-labeled self-assembling protein microarray of new conception, called nucleic acid programmable protein array (NAPPA), by DNASER and by bioinformatics. The DNASER is a novel bioinstrumentation for real-time acquisition and elaboration of images from fluorescent DNA and protein microarrays that permits to acquire images faster than the traditional systems. The fluorescent microarrays images are processed to recognize the DNA and/or protein spots, to analyze their superficial distribution on the glass slide and to evaluate their geometric and intensity properties. NAPPA is finally here utilized in conjunction with a bioinformatic analysis in order to predict protein interactions relevant to progressing between phases of cell cycle of human T lymphocytes and to compare between lymphoma versus normal T cells. Work is in progress about synchronized mitotic HeLa cells compared to logarithmically growing HeLa cells, and about cAMP reverse transformed versus transformed CHO-K1 cells.

Original languageEnglish (US)
Title of host publicationFunctional Proteomics and Nanotechnology-Based Microarrays
PublisherPan Stanford Publishing Pte. Ltd.
Pages31-59
Number of pages29
ISBN (Print)9789814267762
DOIs
StatePublished - Jun 1 2010

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Sivozhelezov, V., Spera, R., Giacomelli, L., Hainsworth, E., LaBaer, J., Bragazzi, N. N., & Nicolini, C. (2010). Bioinformatics and fluorescence dnaser for NAPPA studies on cell transformation and cell cycle. In Functional Proteomics and Nanotechnology-Based Microarrays (pp. 31-59). Pan Stanford Publishing Pte. Ltd.. https://doi.org/10.4032/9789814267779