TY - JOUR
T1 - Biogenic nanoporous silica-based sensor for enhanced electrochemical detection of cardiovascular biomarkers proteins
AU - Lin, Kai Chun
AU - Kunduru, Vindhya
AU - Bothara, Manish
AU - Rege, Kaushal
AU - Prasad, Shalini
AU - Ramakrishna, B. L.
PY - 2010/6
Y1 - 2010/6
N2 - The goal of our research is to demonstrate the feasibility of employing biogenic nanoporous silica as a key component in developing a biosensor platform for rapid label-free electrochemical detection of cardiovascular biomarkers from pure and commercial human serum samples with high sensitivity and selectivity. The biosensor platform consists of a silicon chip with an array of gold electrodes forming multiple sensor sites and works on the principle of electrochemical impedance spectroscopy. Each sensor site is overlaid with a biogenic nanoporous silica membrane that forms a high density of nanowells on top of each electrode. When specific protein biomarkers: C-reactive protein (CRP) and myeloperoxidase (MPO) from a test sample bind to antibodies conjugated to the surface of the gold surface at the base of each nanowell, a perturbation of electrical double layer occurs resulting in a change in the impedance. The performance of the biogenic silica membrane biosensor was tested in comparison with nanoporous alumina membrane-based biosensor and plain metallic thin film biosensor. Significant enhancement in the sensitivity and selectivity was achieved with the biogenic silica biosensor, in comparison to the other two, for detecting the two protein biomarkers from both pure and commercial human serum samples. The sensitivity of the biogenic silica biosensor is ∼1 pg/ml and the linear dose response is observed over a large dynamic range from 1 pg/ml to 1 μg/ml. Based on its performance metrics, the biogenic silica biosensor has excellent potential for development as a point of care handheld electronic biosensor device for detection of protein biomarkers from clinical samples.
AB - The goal of our research is to demonstrate the feasibility of employing biogenic nanoporous silica as a key component in developing a biosensor platform for rapid label-free electrochemical detection of cardiovascular biomarkers from pure and commercial human serum samples with high sensitivity and selectivity. The biosensor platform consists of a silicon chip with an array of gold electrodes forming multiple sensor sites and works on the principle of electrochemical impedance spectroscopy. Each sensor site is overlaid with a biogenic nanoporous silica membrane that forms a high density of nanowells on top of each electrode. When specific protein biomarkers: C-reactive protein (CRP) and myeloperoxidase (MPO) from a test sample bind to antibodies conjugated to the surface of the gold surface at the base of each nanowell, a perturbation of electrical double layer occurs resulting in a change in the impedance. The performance of the biogenic silica membrane biosensor was tested in comparison with nanoporous alumina membrane-based biosensor and plain metallic thin film biosensor. Significant enhancement in the sensitivity and selectivity was achieved with the biogenic silica biosensor, in comparison to the other two, for detecting the two protein biomarkers from both pure and commercial human serum samples. The sensitivity of the biogenic silica biosensor is ∼1 pg/ml and the linear dose response is observed over a large dynamic range from 1 pg/ml to 1 μg/ml. Based on its performance metrics, the biogenic silica biosensor has excellent potential for development as a point of care handheld electronic biosensor device for detection of protein biomarkers from clinical samples.
KW - Biogenic silica biosensor
KW - Cardiovascular disease
KW - Electrochemical impedance spectroscopy
KW - Protein biomarker detection
UR - http://www.scopus.com/inward/record.url?scp=77952581771&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77952581771&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2010.03.032
DO - 10.1016/j.bios.2010.03.032
M3 - Article
C2 - 20417087
AN - SCOPUS:77952581771
SN - 0956-5663
VL - 25
SP - 2336
EP - 2342
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 10
ER -