Biofluid pretreatment using gradient insulator-based dielectrophoresis: separating cells from biomarkers

Jie Ding, Christine Woolley, Mark Hayes

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Blood is one of the most important biofluids used for clinical diagnostics. Cells and proteins in the blood can provide a rich source of information for the evaluation of human health. Efficient separation of blood components is a necessary process in order to minimize the interference of unwanted components during sensing, separation, and detection. In this paper, an insulator-based gradient dielectrophoretic device has been applied to separate red blood cells from model protein biomarkers for myocardial infarction in buffer. Within one min, red blood cells are largely depleted regardless of the minimum adherence on the channel wall. Considering the adhered red blood cells will not be transported further, a purified protein solution can be delivered for potential downstream processing or detection. [Figure not available: see fulltext.]

    Original languageEnglish (US)
    Pages (from-to)1-10
    Number of pages10
    JournalAnalytical and Bioanalytical Chemistry
    DOIs
    StateAccepted/In press - Aug 29 2017

    Fingerprint

    Biomarkers
    Electrophoresis
    Blood
    Erythrocytes
    Cells
    Blood Proteins
    Buffers
    Proteins
    Myocardial Infarction
    Equipment and Supplies
    Health
    Processing

    Keywords

    • Dielectrophoresis
    • Myocardial infarction
    • Protein biomarker
    • Purification
    • Red blood cell
    • Separation

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry

    Cite this

    Biofluid pretreatment using gradient insulator-based dielectrophoresis : separating cells from biomarkers. / Ding, Jie; Woolley, Christine; Hayes, Mark.

    In: Analytical and Bioanalytical Chemistry, 29.08.2017, p. 1-10.

    Research output: Contribution to journalArticle

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