Biodiversity and dynamics of cyanobacterial communities during blooms in temperate lake (Harsha Lake, Ohio, USA)

Bo Zhu, Huansheng Cao, Gaoyang Li, Wei Du, Guangyu Xu, Jorge Santo Domingo, Haiwei Gu, Ning Xu, Shunshan Duan, Jingrang Lu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cyanobacterial blooms are intensifying global ecological hazards. The fine structure and dynamics of bloom community are critical to understanding bloom development but little understood. Here, the questions whether dominant bloomers have high diversity and whether dominant OTUs (operational taxonomical units) compete with one another were addressed. 16S rRNA gene amplicons from an annual bloom at five locations in Harsha Lake (Ohio, USA) showed cyanobacteria were the dominant phylum, and co-existing major bacterial phyla included Proteobacteria, Bacteroidetes, Actinoacteria, and Verrucomicrobia. On the genus level, the initial dominance by Dolichospermum in June yielded to Planktothrix in July, which were replaced by Microcystis and Cylindrospermopsis in August throughout the bloom. Based on the number of verified unique OTUs (a within-genus biodiversity metric), dominant genera tended to have high within-genus diversity. For example, Dolichospermum had 57 unique OTUs, Planktothrix had 36, Microcystis had 12, and Cylindrospermopsis had 4 unique OTUs. Interestingly, these different OTUs showed different dynamics and association with other OTUs. First, no between-OTU competitions were observed during the bloom cycle, and dominant OTUs were abundant throughout the bloom. Such biodiversity of OTUs and their dynamics were verified in Microcystis aeruginosa with two microcystin synthetase genes (mcyA and mcyG): the relative abundance of both genes varied during the bloom based on quantitative PCR. Two Dolichospermum circinale OTUs and one P. rubescens OTU were most abundant and persistently present throughout the entire bloom. Second, these OTUs differed in the OTUs they were associated with. Third, these OTUs tended to have different levels of association with the environmental factors, even they belonged to the same genera. These findings suggest the structure and dynamics of a cyanobacterial bloom community is complex, with only few OTUs dominating the bloom. Thus, high-resolution molecular characterization will be necessary to understand bloom development.

Original languageEnglish (US)
Pages (from-to)9-18
Number of pages10
JournalHarmful Algae
Volume82
DOIs
StatePublished - Feb 1 2019

Fingerprint

algal bloom
Cylindrospermopsis
Planktothrix
biodiversity
lakes
Microcystis
lake
microcystins
Microcystis aeruginosa
genes
Proteobacteria
ligases
Cyanobacteria
quantitative polymerase chain reaction
ribosomal RNA
environmental factors
gene
cyanobacterium
relative abundance
environmental factor

Keywords

  • 16S rRNA gene
  • Cyanobacterial blooms
  • Dolichospermum
  • Genetic diversity
  • Harsha Lake
  • Unique operational taxonomic unit

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

Biodiversity and dynamics of cyanobacterial communities during blooms in temperate lake (Harsha Lake, Ohio, USA). / Zhu, Bo; Cao, Huansheng; Li, Gaoyang; Du, Wei; Xu, Guangyu; Domingo, Jorge Santo; Gu, Haiwei; Xu, Ning; Duan, Shunshan; Lu, Jingrang.

In: Harmful Algae, Vol. 82, 01.02.2019, p. 9-18.

Research output: Contribution to journalArticle

Zhu, Bo ; Cao, Huansheng ; Li, Gaoyang ; Du, Wei ; Xu, Guangyu ; Domingo, Jorge Santo ; Gu, Haiwei ; Xu, Ning ; Duan, Shunshan ; Lu, Jingrang. / Biodiversity and dynamics of cyanobacterial communities during blooms in temperate lake (Harsha Lake, Ohio, USA). In: Harmful Algae. 2019 ; Vol. 82. pp. 9-18.
@article{e6d38cd38a27491194141e75c3e70791,
title = "Biodiversity and dynamics of cyanobacterial communities during blooms in temperate lake (Harsha Lake, Ohio, USA)",
abstract = "Cyanobacterial blooms are intensifying global ecological hazards. The fine structure and dynamics of bloom community are critical to understanding bloom development but little understood. Here, the questions whether dominant bloomers have high diversity and whether dominant OTUs (operational taxonomical units) compete with one another were addressed. 16S rRNA gene amplicons from an annual bloom at five locations in Harsha Lake (Ohio, USA) showed cyanobacteria were the dominant phylum, and co-existing major bacterial phyla included Proteobacteria, Bacteroidetes, Actinoacteria, and Verrucomicrobia. On the genus level, the initial dominance by Dolichospermum in June yielded to Planktothrix in July, which were replaced by Microcystis and Cylindrospermopsis in August throughout the bloom. Based on the number of verified unique OTUs (a within-genus biodiversity metric), dominant genera tended to have high within-genus diversity. For example, Dolichospermum had 57 unique OTUs, Planktothrix had 36, Microcystis had 12, and Cylindrospermopsis had 4 unique OTUs. Interestingly, these different OTUs showed different dynamics and association with other OTUs. First, no between-OTU competitions were observed during the bloom cycle, and dominant OTUs were abundant throughout the bloom. Such biodiversity of OTUs and their dynamics were verified in Microcystis aeruginosa with two microcystin synthetase genes (mcyA and mcyG): the relative abundance of both genes varied during the bloom based on quantitative PCR. Two Dolichospermum circinale OTUs and one P. rubescens OTU were most abundant and persistently present throughout the entire bloom. Second, these OTUs differed in the OTUs they were associated with. Third, these OTUs tended to have different levels of association with the environmental factors, even they belonged to the same genera. These findings suggest the structure and dynamics of a cyanobacterial bloom community is complex, with only few OTUs dominating the bloom. Thus, high-resolution molecular characterization will be necessary to understand bloom development.",
keywords = "16S rRNA gene, Cyanobacterial blooms, Dolichospermum, Genetic diversity, Harsha Lake, Unique operational taxonomic unit",
author = "Bo Zhu and Huansheng Cao and Gaoyang Li and Wei Du and Guangyu Xu and Domingo, {Jorge Santo} and Haiwei Gu and Ning Xu and Shunshan Duan and Jingrang Lu",
year = "2019",
month = "2",
day = "1",
doi = "10.1016/j.hal.2018.12.006",
language = "English (US)",
volume = "82",
pages = "9--18",
journal = "Harmful Algae",
issn = "1568-9883",
publisher = "Elsevier",

}

TY - JOUR

T1 - Biodiversity and dynamics of cyanobacterial communities during blooms in temperate lake (Harsha Lake, Ohio, USA)

AU - Zhu, Bo

AU - Cao, Huansheng

AU - Li, Gaoyang

AU - Du, Wei

AU - Xu, Guangyu

AU - Domingo, Jorge Santo

AU - Gu, Haiwei

AU - Xu, Ning

AU - Duan, Shunshan

AU - Lu, Jingrang

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Cyanobacterial blooms are intensifying global ecological hazards. The fine structure and dynamics of bloom community are critical to understanding bloom development but little understood. Here, the questions whether dominant bloomers have high diversity and whether dominant OTUs (operational taxonomical units) compete with one another were addressed. 16S rRNA gene amplicons from an annual bloom at five locations in Harsha Lake (Ohio, USA) showed cyanobacteria were the dominant phylum, and co-existing major bacterial phyla included Proteobacteria, Bacteroidetes, Actinoacteria, and Verrucomicrobia. On the genus level, the initial dominance by Dolichospermum in June yielded to Planktothrix in July, which were replaced by Microcystis and Cylindrospermopsis in August throughout the bloom. Based on the number of verified unique OTUs (a within-genus biodiversity metric), dominant genera tended to have high within-genus diversity. For example, Dolichospermum had 57 unique OTUs, Planktothrix had 36, Microcystis had 12, and Cylindrospermopsis had 4 unique OTUs. Interestingly, these different OTUs showed different dynamics and association with other OTUs. First, no between-OTU competitions were observed during the bloom cycle, and dominant OTUs were abundant throughout the bloom. Such biodiversity of OTUs and their dynamics were verified in Microcystis aeruginosa with two microcystin synthetase genes (mcyA and mcyG): the relative abundance of both genes varied during the bloom based on quantitative PCR. Two Dolichospermum circinale OTUs and one P. rubescens OTU were most abundant and persistently present throughout the entire bloom. Second, these OTUs differed in the OTUs they were associated with. Third, these OTUs tended to have different levels of association with the environmental factors, even they belonged to the same genera. These findings suggest the structure and dynamics of a cyanobacterial bloom community is complex, with only few OTUs dominating the bloom. Thus, high-resolution molecular characterization will be necessary to understand bloom development.

AB - Cyanobacterial blooms are intensifying global ecological hazards. The fine structure and dynamics of bloom community are critical to understanding bloom development but little understood. Here, the questions whether dominant bloomers have high diversity and whether dominant OTUs (operational taxonomical units) compete with one another were addressed. 16S rRNA gene amplicons from an annual bloom at five locations in Harsha Lake (Ohio, USA) showed cyanobacteria were the dominant phylum, and co-existing major bacterial phyla included Proteobacteria, Bacteroidetes, Actinoacteria, and Verrucomicrobia. On the genus level, the initial dominance by Dolichospermum in June yielded to Planktothrix in July, which were replaced by Microcystis and Cylindrospermopsis in August throughout the bloom. Based on the number of verified unique OTUs (a within-genus biodiversity metric), dominant genera tended to have high within-genus diversity. For example, Dolichospermum had 57 unique OTUs, Planktothrix had 36, Microcystis had 12, and Cylindrospermopsis had 4 unique OTUs. Interestingly, these different OTUs showed different dynamics and association with other OTUs. First, no between-OTU competitions were observed during the bloom cycle, and dominant OTUs were abundant throughout the bloom. Such biodiversity of OTUs and their dynamics were verified in Microcystis aeruginosa with two microcystin synthetase genes (mcyA and mcyG): the relative abundance of both genes varied during the bloom based on quantitative PCR. Two Dolichospermum circinale OTUs and one P. rubescens OTU were most abundant and persistently present throughout the entire bloom. Second, these OTUs differed in the OTUs they were associated with. Third, these OTUs tended to have different levels of association with the environmental factors, even they belonged to the same genera. These findings suggest the structure and dynamics of a cyanobacterial bloom community is complex, with only few OTUs dominating the bloom. Thus, high-resolution molecular characterization will be necessary to understand bloom development.

KW - 16S rRNA gene

KW - Cyanobacterial blooms

KW - Dolichospermum

KW - Genetic diversity

KW - Harsha Lake

KW - Unique operational taxonomic unit

UR - http://www.scopus.com/inward/record.url?scp=85059196867&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85059196867&partnerID=8YFLogxK

U2 - 10.1016/j.hal.2018.12.006

DO - 10.1016/j.hal.2018.12.006

M3 - Article

VL - 82

SP - 9

EP - 18

JO - Harmful Algae

JF - Harmful Algae

SN - 1568-9883

ER -