TY - JOUR
T1 - Biodegradation of single-walled carbon nanotubes by eosinophil peroxidase
AU - Andón, Fernando T.
AU - Kapralov, Alexandr A.
AU - Yanamala, Naveena
AU - Feng, Weihong
AU - Baygan, Arjang
AU - Chambers, Benedict J.
AU - Hultenby, Kjell
AU - Ye, Fei
AU - Toprak, Muhammet S.
AU - Brandner, Birgit D.
AU - Fornara, Andrea
AU - Klein-Seetharaman, Judith
AU - Kotchey, Gregg P.
AU - Star, Alexander
AU - Shvedova, Anna A.
AU - Fadeel, Bengt
AU - Kagan, Valerian E.
N1 - Publisher Copyright:
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2013/8/26
Y1 - 2013/8/26
N2 - Eosinophil peroxidase (EPO) is one of the major oxidant-producing enzymes during inflammatory states in the human lung. The degradation of single-walled carbon nanotubes (SWCNTs) upon incubation with human EPO and H₂O₂ is reported. Biodegradation of SWCNTs is higher in the presence of NaBr, but neither EPO alone nor H₂O₂ alone caused the degradation of nanotubes. Molecular modeling reveals two binding sites for SWCNTs on EPO, one located at the proximal side (same side as the catalytic site) and the other on the distal side of EPO. The oxidized groups on SWCNTs in both cases are stabilized by electrostatic interactions with positively charged residues. Biodegradation of SWCNTs can also be executed in an ex vivo culture system using primary murine eosinophils stimulated to undergo degranulation. Biodegradation is proven by a range of methods including transmission electron microscopy, UV-visible-NIR spectroscopy, Raman spectroscopy, and confocal Raman imaging. Thus, human EPO (in vitro) and ex vivo activated eosinophils mediate biodegradation of SWCNTs: an observation that is relevant to pulmonary responses to these materials.
AB - Eosinophil peroxidase (EPO) is one of the major oxidant-producing enzymes during inflammatory states in the human lung. The degradation of single-walled carbon nanotubes (SWCNTs) upon incubation with human EPO and H₂O₂ is reported. Biodegradation of SWCNTs is higher in the presence of NaBr, but neither EPO alone nor H₂O₂ alone caused the degradation of nanotubes. Molecular modeling reveals two binding sites for SWCNTs on EPO, one located at the proximal side (same side as the catalytic site) and the other on the distal side of EPO. The oxidized groups on SWCNTs in both cases are stabilized by electrostatic interactions with positively charged residues. Biodegradation of SWCNTs can also be executed in an ex vivo culture system using primary murine eosinophils stimulated to undergo degranulation. Biodegradation is proven by a range of methods including transmission electron microscopy, UV-visible-NIR spectroscopy, Raman spectroscopy, and confocal Raman imaging. Thus, human EPO (in vitro) and ex vivo activated eosinophils mediate biodegradation of SWCNTs: an observation that is relevant to pulmonary responses to these materials.
KW - biodegradable materials
KW - carbon nanotubes
KW - eosinophil peroxidase
KW - molecular modeling
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U2 - 10.1002/smll.201202508
DO - 10.1002/smll.201202508
M3 - Article
C2 - 23447468
AN - SCOPUS:84879786954
SN - 1613-6810
VL - 9
SP - 2720
EP - 2729
JO - Small (Weinheim an der Bergstrasse, Germany)
JF - Small (Weinheim an der Bergstrasse, Germany)
IS - 16
ER -