Biodegradation during contaminant transport in porous media

4. Impact of microbial lag and bacterial cell growth

Susannah Sandrin, Fiona L. Jordan, Raina M. Maier, Mark L. Brusseau

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Miscible-displacement experiments were conducted to examine the impact of microbial lag and bacterial cell growth on the transport of salicylate, a model hydrocarbon compound. The impacts of these processes were examined separately, as well as jointly, to determine their relative effects on biodegradation dynamics. For each experiment, a column was packed with porous medium that was first inoculated with bacteria that contained the NAH plasmid encoding genes for the degradation of naphthalene and salicylate, and then subjected to a step input of salicylate solution. The transport behavior of salicylate was non-steady for all cases examined, and was clearly influenced by a delay (lag) in the onset of biodegradation. This microbial lag, which was consistent with the results of batch experiments, is attributed to the induction and synthesis of the enzymes required for biodegradation of salicylate. The effect of microbial lag on salicylate transport was eliminated by exposing the column to two successive pulses of salicylate, thereby allowing the cells to acclimate to the carbon source during the first pulse. Elimination of microbial lag effects allowed the impact of bacterial growth on salicylate transport to be quantified, which was accomplished by determining a cell mass balance. Conversely, the impact of microbial lag was further investigated by performing a similar double-pulse experiment under no-growth conditions. Significant cell elution was observed and quantified for all conditions/systems. The results of these experiments allowed us to differentiate the effects associated with microbial lag and growth, two coupled processes whose impacts on the biodegradation and transport of contaminants can be difficult to distinguish.

Original languageEnglish (US)
Pages (from-to)225-242
Number of pages18
JournalJournal of Contaminant Hydrology
Volume50
Issue number3-4
DOIs
StatePublished - Aug 14 2001
Externally publishedYes

Fingerprint

salicylate
Salicylates
Cell growth
pollutant transport
Biodegradation
Porous materials
porous medium
biodegradation
Impurities
experiment
Experiments
Gene encoding
Hydrocarbons
naphthalene
plasmid
mass balance
Bacteria
Plasmids
Carbon
hydrocarbon

Keywords

  • Bacteria
  • Biodegradation
  • Contaminant transport
  • Organic compounds

ASJC Scopus subject areas

  • Earth-Surface Processes

Cite this

Biodegradation during contaminant transport in porous media : 4. Impact of microbial lag and bacterial cell growth. / Sandrin, Susannah; Jordan, Fiona L.; Maier, Raina M.; Brusseau, Mark L.

In: Journal of Contaminant Hydrology, Vol. 50, No. 3-4, 14.08.2001, p. 225-242.

Research output: Contribution to journalArticle

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