Biochemical characterization of related microtubule proteins in Drosophila melanogaster and adult rat brain

Shaila Srinivasan, Timothy L. Karr

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

We describe the biochemical characteristics of three proteins isolated from Drosophila embryos and the rat brain. We refer to these proteins as DMAPs (Drosophila microtubule-associated proteins) since they were identified by monoclonal antibodies generated against microtubule protein (MTP) purified from Drosophila melanogaster embryos. DMAP-45 is a 45 kDa protein that binds microtubules in an ATP dependent manner. Preliminary biochemical evidence suggests that DMAP-45 may be an actin-related protein. DMAP-55 is a 55 kDa protein and based on its molecular weight and isoelectric point, may be a novel isoform of tubulin. DMAP-66 is a 66 kDa protein that binds strongly to microtubules in vitro and has multiple isoforms. Analyses of proteins in rat brain tissue extracts and purified rat brain MTP identified proteins of similar molecular weight and isoelectric points and are designated DMAP-45R, -55R and -66R. The presence of proteins with common biochemical properties in these widely divergent animal species suggests that they are related proteins.

Original languageEnglish (US)
Pages (from-to)39-46
Number of pages8
JournalBrain Research
Volume701
Issue number1-2
DOIs
StatePublished - Dec 1 1995
Externally publishedYes

Keywords

  • Actin-related proteins
  • Drosophila
  • Microtubule-associated proteins
  • Tubulin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Biochemical characterization of related microtubule proteins in Drosophila melanogaster and adult rat brain'. Together they form a unique fingerprint.

  • Cite this