TY - JOUR
T1 - Biochemical and immunological characterization of the plant-derived candidate human immunodeficiency virus type 1 mucosal vaccine CTB-MPR 649-684
AU - Matoba, Nobuyuki
AU - Kajiura, Hiroyuki
AU - Cherni, Irene
AU - Doran, Jeffrey D.
AU - Bomsel, Morgane
AU - Fujiyama, Kazuhito
AU - Leket-Mor, Tsafrir
PY - 2009/2
Y1 - 2009/2
N2 - Plants are potentially the most economical platforms for the large-scale production of recombinant proteins. Thus, plant-based expression of subunit human immunodeficiency virus type 1 (HIV-1) vaccines provides an opportunity for their global use against the acquired immunodeficiency syndrome pandemic. CTB-MPR649-684 [CTB, cholera toxin B subunit; MPR, membrane proximal (ectodomain) region of gp41] is an HIV-1 vaccine candidate that has been shown previously to induce antibodies that block a pathway of HIV-1 mucosal transmission. In this article, the molecular characterization of CTB-MPR 649-684 expressed in transgenic Nicotiana benthamiana plants is reported. Virtually all of the CTB-MPR649-684 proteins expressed in the selected line were shown to have assembled into pentameric, GM1 ganglioside-binding complexes. Detailed biochemical analyses on the purified protein revealed that it was N-glycosylated, predominantly with high-mannose-type glycans (more than 75%), as predicted from a consensus asparagine-X-serine/threonine (Asn-X-Ser/Thr) N-glycosylation sequon on the CTB domain and an endoplasmic reticulum retention signal attached at the C-terminus of the fusion protein. Despite this modification, the plant-expressed protein retained the nanomolar affinity to GM1 ganglioside and the critical antigenicity of the MPR649-684 moiety. Furthermore, the protein induced mucosal and serum anti-MPR649-684 antibodies in mice after mucosal prime-systemic boost immunization. Our data indicate that plant-based expression can be a viable alternative for the production of this subunit HIV-1 vaccine candidate.
AB - Plants are potentially the most economical platforms for the large-scale production of recombinant proteins. Thus, plant-based expression of subunit human immunodeficiency virus type 1 (HIV-1) vaccines provides an opportunity for their global use against the acquired immunodeficiency syndrome pandemic. CTB-MPR649-684 [CTB, cholera toxin B subunit; MPR, membrane proximal (ectodomain) region of gp41] is an HIV-1 vaccine candidate that has been shown previously to induce antibodies that block a pathway of HIV-1 mucosal transmission. In this article, the molecular characterization of CTB-MPR 649-684 expressed in transgenic Nicotiana benthamiana plants is reported. Virtually all of the CTB-MPR649-684 proteins expressed in the selected line were shown to have assembled into pentameric, GM1 ganglioside-binding complexes. Detailed biochemical analyses on the purified protein revealed that it was N-glycosylated, predominantly with high-mannose-type glycans (more than 75%), as predicted from a consensus asparagine-X-serine/threonine (Asn-X-Ser/Thr) N-glycosylation sequon on the CTB domain and an endoplasmic reticulum retention signal attached at the C-terminus of the fusion protein. Despite this modification, the plant-expressed protein retained the nanomolar affinity to GM1 ganglioside and the critical antigenicity of the MPR649-684 moiety. Furthermore, the protein induced mucosal and serum anti-MPR649-684 antibodies in mice after mucosal prime-systemic boost immunization. Our data indicate that plant-based expression can be a viable alternative for the production of this subunit HIV-1 vaccine candidate.
KW - HIV vaccine
KW - N-glycosylation
KW - Post-translational modification
KW - Transgenic plants
KW - gp41 membrane proximal region
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U2 - 10.1111/j.1467-7652.2008.00381.x
DO - 10.1111/j.1467-7652.2008.00381.x
M3 - Article
C2 - 19037902
AN - SCOPUS:58149387540
SN - 1467-7644
VL - 7
SP - 129
EP - 145
JO - Plant Biotechnology Journal
JF - Plant Biotechnology Journal
IS - 2
ER -