Bioactive surface for neural electrodes: Decreasing astrocyte proliferation via transforming growth factor-β1

Christopher L. Klaver, Michael Caplan

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Implantation of deep-brain recording devices is a traumatic event, which inevitably elicits reactive gliosis. The ensuing glial scar encapsulating the implanted device impedes the long-term functional recording capability of the microelectrode. In this work, a bioactive surface is prepared by conjugation of transforming growth factor-beta one (TGF-β1) and laminin to dextran, which is in turn conjugated to a biomaterial substrate. Poly-L-lysine coated surfaces are treated with oxidized dextran, and the dextran is re-oxidized with sodium metaperiodate to generate hemiacetal structures to which TGF-β1 and laminin are covalently bound. Covalent conjugation of the ligand is confirmed by enzyme-linked immunosorbent assay. A primary cell line of astrocytes is incubated on a surface conjugated with laminin and TGF-β1 and a surface only conjugated with laminin. Proliferation on the laminin plus TGF-β1 surface is 57% less (p < 0.002) than the control surface (laminin alone). The results demonstrate that conjugated TGF-β1 retains its efficacy toward astrocyte proliferation and represents a potential strategy for reducing glial scar formation in vivo.

Original languageEnglish (US)
Pages (from-to)1011-1016
Number of pages6
JournalJournal of Biomedical Materials Research - Part A
Volume81
Issue number4
DOIs
StatePublished - Jun 15 2007

Keywords

  • Astrocytes
  • Biomaterial
  • Cell proliferation inhibition
  • Gliosis
  • Transforming growth factor-β1

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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