Abstract
Implantation of deep-brain recording devices is a traumatic event, which inevitably elicits reactive gliosis. The ensuing glial scar encapsulating the implanted device impedes the long-term functional recording capability of the microelectrode. In this work, a bioactive surface is prepared by conjugation of transforming growth factor-beta one (TGF-β1) and laminin to dextran, which is in turn conjugated to a biomaterial substrate. Poly-L-lysine coated surfaces are treated with oxidized dextran, and the dextran is re-oxidized with sodium metaperiodate to generate hemiacetal structures to which TGF-β1 and laminin are covalently bound. Covalent conjugation of the ligand is confirmed by enzyme-linked immunosorbent assay. A primary cell line of astrocytes is incubated on a surface conjugated with laminin and TGF-β1 and a surface only conjugated with laminin. Proliferation on the laminin plus TGF-β1 surface is 57% less (p < 0.002) than the control surface (laminin alone). The results demonstrate that conjugated TGF-β1 retains its efficacy toward astrocyte proliferation and represents a potential strategy for reducing glial scar formation in vivo.
Original language | English (US) |
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Pages (from-to) | 1011-1016 |
Number of pages | 6 |
Journal | Journal of Biomedical Materials Research - Part A |
Volume | 81 |
Issue number | 4 |
DOIs | |
State | Published - Jun 15 2007 |
Keywords
- Astrocytes
- Biomaterial
- Cell proliferation inhibition
- Gliosis
- Transforming growth factor-β1
ASJC Scopus subject areas
- Ceramics and Composites
- Biomaterials
- Biomedical Engineering
- Metals and Alloys