Bioactive surface for neural electrodes: Decreasing astrocyte proliferation via transforming growth factor-β1

Christopher L. Klaver, Michael Caplan

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Implantation of deep-brain recording devices is a traumatic event, which inevitably elicits reactive gliosis. The ensuing glial scar encapsulating the implanted device impedes the long-term functional recording capability of the microelectrode. In this work, a bioactive surface is prepared by conjugation of transforming growth factor-beta one (TGF-β1) and laminin to dextran, which is in turn conjugated to a biomaterial substrate. Poly-L-lysine coated surfaces are treated with oxidized dextran, and the dextran is re-oxidized with sodium metaperiodate to generate hemiacetal structures to which TGF-β1 and laminin are covalently bound. Covalent conjugation of the ligand is confirmed by enzyme-linked immunosorbent assay. A primary cell line of astrocytes is incubated on a surface conjugated with laminin and TGF-β1 and a surface only conjugated with laminin. Proliferation on the laminin plus TGF-β1 surface is 57% less (p < 0.002) than the control surface (laminin alone). The results demonstrate that conjugated TGF-β1 retains its efficacy toward astrocyte proliferation and represents a potential strategy for reducing glial scar formation in vivo.

Original languageEnglish (US)
Pages (from-to)1011-1016
Number of pages6
JournalJournal of Biomedical Materials Research - Part A
Volume81
Issue number4
DOIs
StatePublished - Jun 15 2007

Fingerprint

Transforming Growth Factors
Laminin
Dextran
Electrodes
Dextrans
Immunosorbents
Control surfaces
Microelectrodes
Biocompatible Materials
Biomaterials
Transforming Growth Factor beta
Lysine
Assays
Brain
Enzymes
Ligands
Cells
Sodium
Astrocytes
Intercellular Signaling Peptides and Proteins

Keywords

  • Astrocytes
  • Biomaterial
  • Cell proliferation inhibition
  • Gliosis
  • Transforming growth factor-β1

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Bioactive surface for neural electrodes : Decreasing astrocyte proliferation via transforming growth factor-β1. / Klaver, Christopher L.; Caplan, Michael.

In: Journal of Biomedical Materials Research - Part A, Vol. 81, No. 4, 15.06.2007, p. 1011-1016.

Research output: Contribution to journalArticle

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