Bioactive isomalabaricane triterpenoids from Rhabdastrella globostellata that stabilize the binding of DNA polymerase β to DNA

Jason A. Clement, Mei Li, Sidney M. Hecht, David G.I. Kingston

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Four isomalabaricane triterpenoids were isolated from an extract of the sponge Rhabdastrella globostellata that was active in an assay measuring stabilization of the binding of DNA with DNA polymerase β. The known compounds stelliferin riboside (1) and 3-epi-29-acetoxystelliferin E (2) were shown to induce 29% and 23% binding, respectively, at 28 μg/mL, while the new compound stellettin J (3) induced 5% binding at 28 μg/mL. The new compound stellettin K (4) had no activity in the binding assay. The compounds were characterized by spectroscopic methods. These compounds displayed varying levels of activity toward the A2780 ovarian cancer cell line, revealing structure-based effects on both the level of cytotoxicity and DNA-polymerase β binding. This is the first report of natural products with the ability to promote stabilization of the DNA-polymerase β covalent binary complex.

Original languageEnglish (US)
Pages (from-to)373-376
Number of pages4
JournalJournal of Natural Products
Volume69
Issue number3
DOIs
StatePublished - Mar 2006

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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