TY - JOUR
T1 - Binding of ferredoxin to algal photosystem I involves a single binding site and is composed of two thermodynamically distinct events
AU - Marco, Pini
AU - Kozuleva, Marina
AU - Eilenberg, Haviva
AU - Mazor, Yuval
AU - Gimeson, Peter
AU - Kanygin, Andrey
AU - Redding, Kevin
AU - Weiner, Iddo
AU - Yacoby, Iftach
N1 - Funding Information:
This study was supported in part by:
Funding Information:
Pini Marco was supported in part by a scholarship from the TAU president renewable energy center.
Funding Information:
ISF personal grant 1646/16
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/4
Y1 - 2018/4
N2 - Despite the impressive progress made in recent years in understanding the early steps in charge separation within the photosynthetic reaction centers, our knowledge of how ferredoxin (Fd) interacts with the acceptor side of photosystem I (PSI) is not as well developed. Fd accepts electrons after transiently docking to a binding site on the acceptor side of PSI. However, the exact location, as well as the stoichiometry, of this binding have been a matter of debate for more than two decades. Here, using Isothermal Titration Calorimetry (ITC) and purified components from wild type and mutant strains of the green algae Chlamydomonas reinhardtii we show that PSI has a single binding site for Fd, and that the association consists of two distinct binding events, each with a specific association constant.
AB - Despite the impressive progress made in recent years in understanding the early steps in charge separation within the photosynthetic reaction centers, our knowledge of how ferredoxin (Fd) interacts with the acceptor side of photosystem I (PSI) is not as well developed. Fd accepts electrons after transiently docking to a binding site on the acceptor side of PSI. However, the exact location, as well as the stoichiometry, of this binding have been a matter of debate for more than two decades. Here, using Isothermal Titration Calorimetry (ITC) and purified components from wild type and mutant strains of the green algae Chlamydomonas reinhardtii we show that PSI has a single binding site for Fd, and that the association consists of two distinct binding events, each with a specific association constant.
KW - Chlamydomonas reinhardtii
KW - Ferredoxin
KW - Isothermal titration calorimetry
KW - Photosystem I
KW - Protein-protein interactions
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U2 - 10.1016/j.bbabio.2018.01.001
DO - 10.1016/j.bbabio.2018.01.001
M3 - Article
C2 - 29337190
AN - SCOPUS:85041582402
SN - 0005-2728
VL - 1859
SP - 234
EP - 243
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
IS - 4
ER -