Binding of ferredoxin to algal photosystem I involves a single binding site and is composed of two thermodynamically distinct events

Pini Marco, Marina Kozuleva, Haviva Eilenberg, Yuval Mazor, Peter Gimeson, Andrey Kanygin, Kevin Redding, Iddo Weiner, Iftach Yacoby

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Despite the impressive progress made in recent years in understanding the early steps in charge separation within the photosynthetic reaction centers, our knowledge of how ferredoxin (Fd) interacts with the acceptor side of photosystem I (PSI) is not as well developed. Fd accepts electrons after transiently docking to a binding site on the acceptor side of PSI. However, the exact location, as well as the stoichiometry, of this binding have been a matter of debate for more than two decades. Here, using Isothermal Titration Calorimetry (ITC) and purified components from wild type and mutant strains of the green algae Chlamydomonas reinhardtii we show that PSI has a single binding site for Fd, and that the association consists of two distinct binding events, each with a specific association constant.

    Original languageEnglish (US)
    Pages (from-to)234-243
    Number of pages10
    JournalBiochimica et Biophysica Acta - Bioenergetics
    Volume1859
    Issue number4
    DOIs
    StatePublished - Apr 1 2018

    Fingerprint

    Photosystem I Protein Complex
    Ferredoxins
    Binding Sites
    Association reactions
    Photosynthetic Reaction Center Complex Proteins
    Chlamydomonas reinhardtii
    Calorimetry
    Chlorophyta
    Algae
    Titration
    Stoichiometry
    Electrons

    Keywords

    • Chlamydomonas reinhardtii
    • Ferredoxin
    • Isothermal titration calorimetry
    • Photosystem I
    • Protein-protein interactions

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Cell Biology

    Cite this

    Binding of ferredoxin to algal photosystem I involves a single binding site and is composed of two thermodynamically distinct events. / Marco, Pini; Kozuleva, Marina; Eilenberg, Haviva; Mazor, Yuval; Gimeson, Peter; Kanygin, Andrey; Redding, Kevin; Weiner, Iddo; Yacoby, Iftach.

    In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1859, No. 4, 01.04.2018, p. 234-243.

    Research output: Contribution to journalArticle

    Marco, Pini ; Kozuleva, Marina ; Eilenberg, Haviva ; Mazor, Yuval ; Gimeson, Peter ; Kanygin, Andrey ; Redding, Kevin ; Weiner, Iddo ; Yacoby, Iftach. / Binding of ferredoxin to algal photosystem I involves a single binding site and is composed of two thermodynamically distinct events. In: Biochimica et Biophysica Acta - Bioenergetics. 2018 ; Vol. 1859, No. 4. pp. 234-243.
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    abstract = "Despite the impressive progress made in recent years in understanding the early steps in charge separation within the photosynthetic reaction centers, our knowledge of how ferredoxin (Fd) interacts with the acceptor side of photosystem I (PSI) is not as well developed. Fd accepts electrons after transiently docking to a binding site on the acceptor side of PSI. However, the exact location, as well as the stoichiometry, of this binding have been a matter of debate for more than two decades. Here, using Isothermal Titration Calorimetry (ITC) and purified components from wild type and mutant strains of the green algae Chlamydomonas reinhardtii we show that PSI has a single binding site for Fd, and that the association consists of two distinct binding events, each with a specific association constant.",
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    AU - Marco, Pini

    AU - Kozuleva, Marina

    AU - Eilenberg, Haviva

    AU - Mazor, Yuval

    AU - Gimeson, Peter

    AU - Kanygin, Andrey

    AU - Redding, Kevin

    AU - Weiner, Iddo

    AU - Yacoby, Iftach

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