Binding of ferredoxin to algal photosystem I involves a single binding site and is composed of two thermodynamically distinct events

Pini Marco, Marina Kozuleva, Haviva Eilenberg, Yuval Mazor, Peter Gimeson, Andrey Kanygin, Kevin Redding, Iddo Weiner, Iftach Yacoby

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Despite the impressive progress made in recent years in understanding the early steps in charge separation within the photosynthetic reaction centers, our knowledge of how ferredoxin (Fd) interacts with the acceptor side of photosystem I (PSI) is not as well developed. Fd accepts electrons after transiently docking to a binding site on the acceptor side of PSI. However, the exact location, as well as the stoichiometry, of this binding have been a matter of debate for more than two decades. Here, using Isothermal Titration Calorimetry (ITC) and purified components from wild type and mutant strains of the green algae Chlamydomonas reinhardtii we show that PSI has a single binding site for Fd, and that the association consists of two distinct binding events, each with a specific association constant.

Original languageEnglish (US)
Pages (from-to)234-243
Number of pages10
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1859
Issue number4
DOIs
StatePublished - Apr 1 2018

Fingerprint

Photosystem I Protein Complex
Ferredoxins
Binding Sites
Association reactions
Photosynthetic Reaction Center Complex Proteins
Chlamydomonas reinhardtii
Calorimetry
Chlorophyta
Algae
Titration
Stoichiometry
Electrons

Keywords

  • Chlamydomonas reinhardtii
  • Ferredoxin
  • Isothermal titration calorimetry
  • Photosystem I
  • Protein-protein interactions

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Binding of ferredoxin to algal photosystem I involves a single binding site and is composed of two thermodynamically distinct events. / Marco, Pini; Kozuleva, Marina; Eilenberg, Haviva; Mazor, Yuval; Gimeson, Peter; Kanygin, Andrey; Redding, Kevin; Weiner, Iddo; Yacoby, Iftach.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1859, No. 4, 01.04.2018, p. 234-243.

Research output: Contribution to journalArticle

Marco, Pini ; Kozuleva, Marina ; Eilenberg, Haviva ; Mazor, Yuval ; Gimeson, Peter ; Kanygin, Andrey ; Redding, Kevin ; Weiner, Iddo ; Yacoby, Iftach. / Binding of ferredoxin to algal photosystem I involves a single binding site and is composed of two thermodynamically distinct events. In: Biochimica et Biophysica Acta - Bioenergetics. 2018 ; Vol. 1859, No. 4. pp. 234-243.
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AU - Gimeson, Peter

AU - Kanygin, Andrey

AU - Redding, Kevin

AU - Weiner, Iddo

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