Binding of α-factor pheromone to yeast a cells: Chemical and genetic evidence for an α-factor receptor

Duane D. Jenness, Anne C. Burkholder, Leland H. Hartwell

Research output: Contribution to journalArticle

101 Scopus citations

Abstract

The division cycle of yeast a cells is inhibited by α-factor. Haploid a cells were found to bind 35S-labeled α-factor, whereas haploid α cells and diploid a/α cells showed little binding. The association of α-factor with a cells was reversible upon dilution. Unlabeled α-factor competed for binding of 35S-α-factor; the concentration dependence for competition indicated 9 × 105 binding sites per cell with a dissociation constant (KD) of 3 × 10-7 M. The rates of association (kon = 3 × 103 M-1 sec-1) and dissociation (koff = 9 × 10-4 sec-1) were consistent with the equilibrium constant. The α-factor binding activity associated with five temperature-sensitive ste2 mutants was thermolabile, suggesting that the STE2 gene encodes the receptor for α-factor. In contrast, the binding activity of other temperature-sensitive mutants (ste4, ste5, ste7, ste11, and ste12) showed no thermolability.

Original languageEnglish (US)
Pages (from-to)521-529
Number of pages9
JournalCell
Volume35
Issue number2 PART 1
DOIs
StatePublished - Dec 1983
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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