BIG-TREE: Base-Edited Isogenic hPSC Line Generation Using a Transient Reporter for Editing Enrichment

Nicholas Brookhouser, Stefan J. Tekel, Kylie Standage-Beier, Toan Nguyen, Grace Schwarz, Xiao Wang, David A. Brafman

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Current CRISPR-targeted single-nucleotide modifications and subsequent isogenic cell line generation in human pluripotent stem cells (hPSCs) require the introduction of deleterious double-stranded DNA breaks followed by inefficient homology-directed repair (HDR). Here, we utilize Cas9 deaminase base-editing technologies to co-target genomic loci and an episomal reporter to enable single-nucleotide genomic changes in hPSCs without HDR. Together, this method entitled base-edited isogenic hPSC line generation using a transient reporter for editing enrichment (BIG-TREE) allows for single-nucleotide editing efficiencies of >80% across multiple hPSC lines. In addition, we show that BIG-TREE allows for efficient generation of loss-of-function hPSC lines via introduction of premature stop codons. Finally, we use BIG-TREE to achieve efficient multiplex editing of hPSCs at several independent loci. This easily adoptable method will allow for the precise and efficient base editing of hPSCs for use in developmental biology, disease modeling, drug screening, and cell-based therapies.

Original languageEnglish (US)
Pages (from-to)184-191
Number of pages8
JournalStem Cell Reports
Volume14
Issue number2
DOIs
StatePublished - Feb 11 2020

Keywords

  • CRISPR
  • base editor
  • genome modification
  • human pluripotent stem cells
  • multiplexing

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

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