Bean yellow dwarf virus replicons for high-level transgene expression in transgenic plants and cell cultures

Xiuren Zhang, Hugh Mason

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

A novel stable transgenic plant expression system was developed using elements of the replication machinery of Bean Yellow Dwarf Virus (BeYDV). The system contains two transgenes: 1) The BeYDV replicon vector with an expression cassette flanked by cis-acting DNA elements of BeYDV, and 2) The viral replication initiator protein (Rep) controlled by an alcohol-inducible promoter. When Rep expression was triggered by treatment with ethanol, it induced release of the BeYDV replicon from stably integrated T-DNA and episomal replication to high copy number. Replicon amplification resulted in substantially increased transgene mRNA levels (up to 80-fold) and translation products (up to 10-fold) after induction of Rep expression by ethanol treatment in tobacco NT1 cells and leaves of whole potato plants. Thus, the BeYDV stable transformant replicon system is a powerful tool for plant-based production of recombinant proteins.

Original languageEnglish (US)
Pages (from-to)271-279
Number of pages9
JournalBiotechnology and bioengineering
Volume93
Issue number2
DOIs
StatePublished - Feb 5 2006

Keywords

  • Bean yellow dwarf virus
  • Norwalk virus capsid protein
  • Overexpression
  • Rep
  • Replicon
  • Transgene

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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