Basidioascus undulatus: Genome, origins, and sexuality

Hai D T Nguyen, Denise Chabot, Yuuri Hirooka, Robert Roberson, Keith A. Seifert

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Basidioascus undulatus is a soil basidiomycete belonging to the order Geminibasidiales. The taxonomic status of the order was unclear as originally it was only tentatively classified in the class Wallemiomycetes. The fungi in Geminibasidiales have an ambiguously defined sexual cycle. In this study, we sequenced the genome of B. undulatus to gain insights into its sexuality and evolutionary origins. The assembled genome draft was approximately 32 Mb in size, had a median nucleotide coverage of 24X, and contained 6123 predicted genes. Previous morphological descriptions of B. undulatus relied on interpretation of putative sexual structures. In this study, nuclear staining and confocal microscopy showed meiosis occurring in basidia and genome analysis confirmed the existence of genes involved in meiosis and mating. Using 35 protein-coding genes extracted from genomic information, phylogenomic and molecular dating analyses confirmed that B. undulatus indeed belongs to a lineage distantly related to Wallemia while retaining a basal position in Agaricomycotina. These results, combined with differences in septal pore morphology, led us to move the order Geminibasidiales out of the Wallemiomycetes and into the new class Geminibasidiomycetes cl. nov. Finally, the concept of Agaricomycotina is emended to include both Wallemiomycetes and Geminibasidiomycetes.

Original languageEnglish (US)
Pages (from-to)215-231
Number of pages17
JournalIMA Fungus
Volume6
Issue number1
DOIs
StatePublished - 2015

Fingerprint

Wallemiomycetes
sexuality
Agaricomycotina
Sexuality
genome
Meiosis
aprepitant
Genome
meiosis
Nuclear Microscopy
gene
Wallemia
basidia
Basidiomycota
genes
Confocal Microscopy
Genes
microscopy
genomics
Fungi

Keywords

  • Agaricomycotina
  • Basidiomycota
  • Geminibasidiomycetes
  • Septal pore ultastructure
  • Wallemiomycetes

ASJC Scopus subject areas

  • Plant Science

Cite this

Nguyen, H. D. T., Chabot, D., Hirooka, Y., Roberson, R., & Seifert, K. A. (2015). Basidioascus undulatus: Genome, origins, and sexuality. IMA Fungus, 6(1), 215-231. https://doi.org/10.5598/imafungus.2015.06.01.14

Basidioascus undulatus : Genome, origins, and sexuality. / Nguyen, Hai D T; Chabot, Denise; Hirooka, Yuuri; Roberson, Robert; Seifert, Keith A.

In: IMA Fungus, Vol. 6, No. 1, 2015, p. 215-231.

Research output: Contribution to journalArticle

Nguyen, HDT, Chabot, D, Hirooka, Y, Roberson, R & Seifert, KA 2015, 'Basidioascus undulatus: Genome, origins, and sexuality', IMA Fungus, vol. 6, no. 1, pp. 215-231. https://doi.org/10.5598/imafungus.2015.06.01.14
Nguyen, Hai D T ; Chabot, Denise ; Hirooka, Yuuri ; Roberson, Robert ; Seifert, Keith A. / Basidioascus undulatus : Genome, origins, and sexuality. In: IMA Fungus. 2015 ; Vol. 6, No. 1. pp. 215-231.
@article{332a899526084b1b80dd0b9207c0a88c,
title = "Basidioascus undulatus: Genome, origins, and sexuality",
abstract = "Basidioascus undulatus is a soil basidiomycete belonging to the order Geminibasidiales. The taxonomic status of the order was unclear as originally it was only tentatively classified in the class Wallemiomycetes. The fungi in Geminibasidiales have an ambiguously defined sexual cycle. In this study, we sequenced the genome of B. undulatus to gain insights into its sexuality and evolutionary origins. The assembled genome draft was approximately 32 Mb in size, had a median nucleotide coverage of 24X, and contained 6123 predicted genes. Previous morphological descriptions of B. undulatus relied on interpretation of putative sexual structures. In this study, nuclear staining and confocal microscopy showed meiosis occurring in basidia and genome analysis confirmed the existence of genes involved in meiosis and mating. Using 35 protein-coding genes extracted from genomic information, phylogenomic and molecular dating analyses confirmed that B. undulatus indeed belongs to a lineage distantly related to Wallemia while retaining a basal position in Agaricomycotina. These results, combined with differences in septal pore morphology, led us to move the order Geminibasidiales out of the Wallemiomycetes and into the new class Geminibasidiomycetes cl. nov. Finally, the concept of Agaricomycotina is emended to include both Wallemiomycetes and Geminibasidiomycetes.",
keywords = "Agaricomycotina, Basidiomycota, Geminibasidiomycetes, Septal pore ultastructure, Wallemiomycetes",
author = "Nguyen, {Hai D T} and Denise Chabot and Yuuri Hirooka and Robert Roberson and Seifert, {Keith A.}",
year = "2015",
doi = "10.5598/imafungus.2015.06.01.14",
language = "English (US)",
volume = "6",
pages = "215--231",
journal = "IMA Fungus",
issn = "2210-6340",
publisher = "International Mycological Association",
number = "1",

}

TY - JOUR

T1 - Basidioascus undulatus

T2 - Genome, origins, and sexuality

AU - Nguyen, Hai D T

AU - Chabot, Denise

AU - Hirooka, Yuuri

AU - Roberson, Robert

AU - Seifert, Keith A.

PY - 2015

Y1 - 2015

N2 - Basidioascus undulatus is a soil basidiomycete belonging to the order Geminibasidiales. The taxonomic status of the order was unclear as originally it was only tentatively classified in the class Wallemiomycetes. The fungi in Geminibasidiales have an ambiguously defined sexual cycle. In this study, we sequenced the genome of B. undulatus to gain insights into its sexuality and evolutionary origins. The assembled genome draft was approximately 32 Mb in size, had a median nucleotide coverage of 24X, and contained 6123 predicted genes. Previous morphological descriptions of B. undulatus relied on interpretation of putative sexual structures. In this study, nuclear staining and confocal microscopy showed meiosis occurring in basidia and genome analysis confirmed the existence of genes involved in meiosis and mating. Using 35 protein-coding genes extracted from genomic information, phylogenomic and molecular dating analyses confirmed that B. undulatus indeed belongs to a lineage distantly related to Wallemia while retaining a basal position in Agaricomycotina. These results, combined with differences in septal pore morphology, led us to move the order Geminibasidiales out of the Wallemiomycetes and into the new class Geminibasidiomycetes cl. nov. Finally, the concept of Agaricomycotina is emended to include both Wallemiomycetes and Geminibasidiomycetes.

AB - Basidioascus undulatus is a soil basidiomycete belonging to the order Geminibasidiales. The taxonomic status of the order was unclear as originally it was only tentatively classified in the class Wallemiomycetes. The fungi in Geminibasidiales have an ambiguously defined sexual cycle. In this study, we sequenced the genome of B. undulatus to gain insights into its sexuality and evolutionary origins. The assembled genome draft was approximately 32 Mb in size, had a median nucleotide coverage of 24X, and contained 6123 predicted genes. Previous morphological descriptions of B. undulatus relied on interpretation of putative sexual structures. In this study, nuclear staining and confocal microscopy showed meiosis occurring in basidia and genome analysis confirmed the existence of genes involved in meiosis and mating. Using 35 protein-coding genes extracted from genomic information, phylogenomic and molecular dating analyses confirmed that B. undulatus indeed belongs to a lineage distantly related to Wallemia while retaining a basal position in Agaricomycotina. These results, combined with differences in septal pore morphology, led us to move the order Geminibasidiales out of the Wallemiomycetes and into the new class Geminibasidiomycetes cl. nov. Finally, the concept of Agaricomycotina is emended to include both Wallemiomycetes and Geminibasidiomycetes.

KW - Agaricomycotina

KW - Basidiomycota

KW - Geminibasidiomycetes

KW - Septal pore ultastructure

KW - Wallemiomycetes

UR - http://www.scopus.com/inward/record.url?scp=84940947119&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84940947119&partnerID=8YFLogxK

U2 - 10.5598/imafungus.2015.06.01.14

DO - 10.5598/imafungus.2015.06.01.14

M3 - Article

AN - SCOPUS:84940947119

VL - 6

SP - 215

EP - 231

JO - IMA Fungus

JF - IMA Fungus

SN - 2210-6340

IS - 1

ER -