Baculovirus display of fusion protein of Peste des petits ruminants virus and hemagglutination protein of Rinderpest virus and immunogenicity of the displayed proteins in mouse model

Md Masmudur Rahman; M. S. Shaila; Karumathil P. Gopinathan

doi:10.1016/j.virol.2003.08.022

Baculovirus display of fusion protein of Peste des petits ruminants virus and hemagglutination protein of Rinderpest virus and immunogenicity of the displayed proteins in mouse model

Md Masmudur Rahman, M. S. Shaila, Karumathil P. Gopinathan

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Recombinant Bombyx mori nucleopolyhedroviruses (BmNPV) displaying the immunodominant ectodomains of fusion glycoprotein (F) of Peste des petitis ruminants virus (PPRV) and the hemagglutinin protein (H) of Rinderpest virus (RPV), on the budded virions as well as the surface of the infected host cells have been constructed. The F and H protein sequences were inserted in-frame within the amino-terminal region of BmNPV envelope glycoprotein GP64 expressing under the strong viral polyhedrin (polh) promoter. We improved the recombinant virus selection in BmNPV by incorporating the green fluorescent protein gene (gfp) as selection marker under a separate promoter within the transfer cassette harboring the desired genes. Following infection of the insect larvae or the host-derived BmN cells with these recombinant BmNPVs, the expressed GP64 fusion proteins were displayed on the host cell surface and the budded virions. The antigenic epitopes of the recombinant proteins were properly displayed and the recombinant virus particles induced immune response in mice against PPRV or RPV.

Original language	English (US)
Pages (from-to)	36-49
Number of pages	14
Journal	Virology
Volume	317
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2003.08.022
State	Published - Dec 5 2003
Externally published	Yes

Keywords

Antigen display
Baculovirus
BmNPV
Bombyx mori
Cell-surface display
Fusion protein
Hemagglutinin protein
Peste des petits ruminants virus
Rinderpest virus
Viral display

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2003.08.022

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@article{1acc765e673c4b09afef2ae341fb0e5a,

title = "Baculovirus display of fusion protein of Peste des petits ruminants virus and hemagglutination protein of Rinderpest virus and immunogenicity of the displayed proteins in mouse model",

abstract = "Recombinant Bombyx mori nucleopolyhedroviruses (BmNPV) displaying the immunodominant ectodomains of fusion glycoprotein (F) of Peste des petitis ruminants virus (PPRV) and the hemagglutinin protein (H) of Rinderpest virus (RPV), on the budded virions as well as the surface of the infected host cells have been constructed. The F and H protein sequences were inserted in-frame within the amino-terminal region of BmNPV envelope glycoprotein GP64 expressing under the strong viral polyhedrin (polh) promoter. We improved the recombinant virus selection in BmNPV by incorporating the green fluorescent protein gene (gfp) as selection marker under a separate promoter within the transfer cassette harboring the desired genes. Following infection of the insect larvae or the host-derived BmN cells with these recombinant BmNPVs, the expressed GP64 fusion proteins were displayed on the host cell surface and the budded virions. The antigenic epitopes of the recombinant proteins were properly displayed and the recombinant virus particles induced immune response in mice against PPRV or RPV.",

keywords = "Antigen display, Baculovirus, BmNPV, Bombyx mori, Cell-surface display, Fusion protein, Hemagglutinin protein, Peste des petits ruminants virus, Rinderpest virus, Viral display",

author = "Rahman, {Md Masmudur} and Shaila, {M. S.} and Gopinathan, {Karumathil P.}",

note = "Funding Information: We thank the Department of Biotechnology, Government of India, and the Indo-French Center for Promotion of Advanced Research (IFCPAR) for financial support; the Institute Divisional facilities for the use of Confocal microscope and FACS scan; and the CSRTI (Central Silk Board), Mysore, for the supply of B. mori larvae. M.M.R. is a recipient of SAARC (South Asian Association for Regional Cooperation) Fellowship under the Indian Council for Cultural Relations Exchange Program, and K.P.G. is CSIR Emeritus Scientist.",

year = "2003",

month = dec,

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doi = "10.1016/j.virol.2003.08.022",

language = "English (US)",

volume = "317",

pages = "36--49",

journal = "Virology",

issn = "0042-6822",

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T1 - Baculovirus display of fusion protein of Peste des petits ruminants virus and hemagglutination protein of Rinderpest virus and immunogenicity of the displayed proteins in mouse model

AU - Rahman, Md Masmudur

AU - Shaila, M. S.

AU - Gopinathan, Karumathil P.

N1 - Funding Information: We thank the Department of Biotechnology, Government of India, and the Indo-French Center for Promotion of Advanced Research (IFCPAR) for financial support; the Institute Divisional facilities for the use of Confocal microscope and FACS scan; and the CSRTI (Central Silk Board), Mysore, for the supply of B. mori larvae. M.M.R. is a recipient of SAARC (South Asian Association for Regional Cooperation) Fellowship under the Indian Council for Cultural Relations Exchange Program, and K.P.G. is CSIR Emeritus Scientist.

PY - 2003/12/5

Y1 - 2003/12/5

N2 - Recombinant Bombyx mori nucleopolyhedroviruses (BmNPV) displaying the immunodominant ectodomains of fusion glycoprotein (F) of Peste des petitis ruminants virus (PPRV) and the hemagglutinin protein (H) of Rinderpest virus (RPV), on the budded virions as well as the surface of the infected host cells have been constructed. The F and H protein sequences were inserted in-frame within the amino-terminal region of BmNPV envelope glycoprotein GP64 expressing under the strong viral polyhedrin (polh) promoter. We improved the recombinant virus selection in BmNPV by incorporating the green fluorescent protein gene (gfp) as selection marker under a separate promoter within the transfer cassette harboring the desired genes. Following infection of the insect larvae or the host-derived BmN cells with these recombinant BmNPVs, the expressed GP64 fusion proteins were displayed on the host cell surface and the budded virions. The antigenic epitopes of the recombinant proteins were properly displayed and the recombinant virus particles induced immune response in mice against PPRV or RPV.

AB - Recombinant Bombyx mori nucleopolyhedroviruses (BmNPV) displaying the immunodominant ectodomains of fusion glycoprotein (F) of Peste des petitis ruminants virus (PPRV) and the hemagglutinin protein (H) of Rinderpest virus (RPV), on the budded virions as well as the surface of the infected host cells have been constructed. The F and H protein sequences were inserted in-frame within the amino-terminal region of BmNPV envelope glycoprotein GP64 expressing under the strong viral polyhedrin (polh) promoter. We improved the recombinant virus selection in BmNPV by incorporating the green fluorescent protein gene (gfp) as selection marker under a separate promoter within the transfer cassette harboring the desired genes. Following infection of the insect larvae or the host-derived BmN cells with these recombinant BmNPVs, the expressed GP64 fusion proteins were displayed on the host cell surface and the budded virions. The antigenic epitopes of the recombinant proteins were properly displayed and the recombinant virus particles induced immune response in mice against PPRV or RPV.

KW - Antigen display

KW - Baculovirus

KW - BmNPV

KW - Bombyx mori

KW - Cell-surface display

KW - Fusion protein

KW - Hemagglutinin protein

KW - Peste des petits ruminants virus

KW - Rinderpest virus

KW - Viral display

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AN - SCOPUS:0345735918

SN - 0042-6822

VL - 317

SP - 36

EP - 49

JO - Virology

JF - Virology

IS - 1

ER -

Baculovirus display of fusion protein of Peste des petits ruminants virus and hemagglutination protein of Rinderpest virus and immunogenicity of the displayed proteins in mouse model

Abstract

Keywords

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