Baculovirus display of fusion protein of Peste des petits ruminants virus and hemagglutination protein of Rinderpest virus and immunogenicity of the displayed proteins in mouse model

Masmudur Rahman, M. S. Shaila, Karumathil P. Gopinathan

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Recombinant Bombyx mori nucleopolyhedroviruses (BmNPV) displaying the immunodominant ectodomains of fusion glycoprotein (F) of Peste des petitis ruminants virus (PPRV) and the hemagglutinin protein (H) of Rinderpest virus (RPV), on the budded virions as well as the surface of the infected host cells have been constructed. The F and H protein sequences were inserted in-frame within the amino-terminal region of BmNPV envelope glycoprotein GP64 expressing under the strong viral polyhedrin (polh) promoter. We improved the recombinant virus selection in BmNPV by incorporating the green fluorescent protein gene (gfp) as selection marker under a separate promoter within the transfer cassette harboring the desired genes. Following infection of the insect larvae or the host-derived BmN cells with these recombinant BmNPVs, the expressed GP64 fusion proteins were displayed on the host cell surface and the budded virions. The antigenic epitopes of the recombinant proteins were properly displayed and the recombinant virus particles induced immune response in mice against PPRV or RPV.

Original languageEnglish (US)
Pages (from-to)36-49
Number of pages14
JournalVirology
Volume317
Issue number1
DOIs
StatePublished - Dec 5 2003
Externally publishedYes

Fingerprint

Peste-des-petits-ruminants virus
Rinderpest virus
Nucleopolyhedrovirus
Bombyx
Baculoviridae
Hemagglutination
Virion
Ruminants
Viruses
Glycoproteins
Proteins
Hemagglutinins
Green Fluorescent Proteins
Recombinant Proteins
Genes
Larva
Insects
Epitopes
Infection

Keywords

  • Antigen display
  • Baculovirus
  • BmNPV
  • Bombyx mori
  • Cell-surface display
  • Fusion protein
  • Hemagglutinin protein
  • Peste des petits ruminants virus
  • Rinderpest virus
  • Viral display

ASJC Scopus subject areas

  • Virology

Cite this

Baculovirus display of fusion protein of Peste des petits ruminants virus and hemagglutination protein of Rinderpest virus and immunogenicity of the displayed proteins in mouse model. / Rahman, Masmudur; Shaila, M. S.; Gopinathan, Karumathil P.

In: Virology, Vol. 317, No. 1, 05.12.2003, p. 36-49.

Research output: Contribution to journalArticle

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