Abstract
Bacteria produce unique volatile mixtures that could be used to identify infectious agents to the species, and possibly the strain level. However, due to the immense variety of human pathogens, and the close relatedness of some of these bacteria, the robust identification of the bacterium based on its volatile metabolome is likely to require a large number of volatile compounds for each species. We applied comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOFMS) to the identification of the headspace volatiles of Pseudomonas aeruginosa PA14 grown for 24. h in lysogeny broth. This is the first reported use of GC × GC-TOFMS for the characterization of bacterial headspace volatiles. The analytical purity that is afforded by this chromatographic method facilitated the identification of 28 new P. aeruginosa-derived volatiles, nearly doubling the list of volatiles for this species.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 41-46 |
| Number of pages | 6 |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 901 |
| DOIs | |
| State | Published - Jul 15 2012 |
| Externally published | Yes |
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Keywords
- Bacterial volatiles
- GC-TOFMS
- Metabolomics
- Pseudomonas aeruginosa
- SPME
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Cell Biology
- Clinical Biochemistry
Cite this
Bacterial volatile discovery using solid phase microextraction and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry. / Bean, Heather; Dimandja, Jean Marie D; Hill, Jane E.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 901, 15.07.2012, p. 41-46.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bacterial volatile discovery using solid phase microextraction and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry
AU - Bean, Heather
AU - Dimandja, Jean Marie D
AU - Hill, Jane E.
PY - 2012/7/15
Y1 - 2012/7/15
N2 - Bacteria produce unique volatile mixtures that could be used to identify infectious agents to the species, and possibly the strain level. However, due to the immense variety of human pathogens, and the close relatedness of some of these bacteria, the robust identification of the bacterium based on its volatile metabolome is likely to require a large number of volatile compounds for each species. We applied comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOFMS) to the identification of the headspace volatiles of Pseudomonas aeruginosa PA14 grown for 24. h in lysogeny broth. This is the first reported use of GC × GC-TOFMS for the characterization of bacterial headspace volatiles. The analytical purity that is afforded by this chromatographic method facilitated the identification of 28 new P. aeruginosa-derived volatiles, nearly doubling the list of volatiles for this species.
AB - Bacteria produce unique volatile mixtures that could be used to identify infectious agents to the species, and possibly the strain level. However, due to the immense variety of human pathogens, and the close relatedness of some of these bacteria, the robust identification of the bacterium based on its volatile metabolome is likely to require a large number of volatile compounds for each species. We applied comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOFMS) to the identification of the headspace volatiles of Pseudomonas aeruginosa PA14 grown for 24. h in lysogeny broth. This is the first reported use of GC × GC-TOFMS for the characterization of bacterial headspace volatiles. The analytical purity that is afforded by this chromatographic method facilitated the identification of 28 new P. aeruginosa-derived volatiles, nearly doubling the list of volatiles for this species.
KW - Bacterial volatiles
KW - GC-TOFMS
KW - Metabolomics
KW - Pseudomonas aeruginosa
KW - SPME
UR - http://www.scopus.com/inward/record.url?scp=84863775347&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863775347&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2012.05.038
DO - 10.1016/j.jchromb.2012.05.038
M3 - Article
VL - 901
SP - 41
EP - 46
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
ER -