Bacterial volatile discovery using solid phase microextraction and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry

Heather Bean, Jean Marie D Dimandja, Jane E. Hill

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Bacteria produce unique volatile mixtures that could be used to identify infectious agents to the species, and possibly the strain level. However, due to the immense variety of human pathogens, and the close relatedness of some of these bacteria, the robust identification of the bacterium based on its volatile metabolome is likely to require a large number of volatile compounds for each species. We applied comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOFMS) to the identification of the headspace volatiles of Pseudomonas aeruginosa PA14 grown for 24. h in lysogeny broth. This is the first reported use of GC × GC-TOFMS for the characterization of bacterial headspace volatiles. The analytical purity that is afforded by this chromatographic method facilitated the identification of 28 new P. aeruginosa-derived volatiles, nearly doubling the list of volatiles for this species.

Original languageEnglish (US)
Pages (from-to)41-46
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume901
DOIs
StatePublished - Jul 15 2012
Externally publishedYes

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Solid Phase Microextraction
Gas chromatography
Gas Chromatography
Mass spectrometry
Mass Spectrometry
Bacteria
Pseudomonas aeruginosa
Lysogeny
Metabolome
Pathogens

Keywords

  • Bacterial volatiles
  • GC-TOFMS
  • Metabolomics
  • Pseudomonas aeruginosa
  • SPME

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

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