Bacillus subtilis endospores at high purity and recovery yields: Optimization of growth conditions and purification method

Milene B. Tavares, Renata D. Souza, Wilson B. Luiz, Rafael C.M. Cavalcante, Caroline Casaroli, Eduardo G. Martins, Rita C.C. Ferreira, Luís C.S. Ferreira

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Bacillus subtilis endospores have applications in different fields including their use as probiotics and antigen delivery vectors. Such specialized applications frequently require highly purified spore preparations. Nonetheless, quantitative data regarding both yields and purity of B. subtilis endospores after application of different growth conditions and purification methods are scarce or poorly reported. In the present study, we conducted several quantitative and qualitative analyses of growth conditions and purification procedures aiming generation of purified B. subtilis spores. Based on two growth media and different incubations conditions, sporulation frequencies up to 74.2 % and spore concentrations up to 7 × 109 spores/ml were achieved. Application of a simplified spore isolation method, in which samples were incubated with lysozyme and a detergent, resulted in preparations with highly purified spores at the highest yields. The present study represents, therefore, an important contribution for those working with B. subtilis endospores for different biotechnological purposes.

Original languageEnglish (US)
Pages (from-to)279-285
Number of pages7
JournalCurrent Microbiology
Volume66
Issue number3
DOIs
StatePublished - Mar 2013

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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