B7-H3 enhances tumor immunity in vivo by costimulating rapid clonal expansion of antigen-specific CD8+ cytolytic T cells

Liqun Luo, Andrei Chapoval, Dallas B. Flies, Gefeng Zhu, Fumiya Hirano, Shengdian Wang, Julie S. Lau, Haidong Dong, Koji Tamada, Andrew S. Flies, Yang Liu, Lieping Chen

Research output: Contribution to journalArticle

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Abstract

B7-H3 is a B7 family molecule with T cell costimulatory function in vitro. The in vivo role of B7-H3 in the stimulation of tumor immunity is unclear. We report here that expression of B7-H3 by transfection of the mouse P815 tumor line enhances its immunogenicity, leading to the regression of tumors and amplification of a tumor-specific CD8+ CTL response in syngeneic mice. Tumor cells engineered to express B7-H3 elicit a rapid clonal expansion of P1A tumor Ag-specific CD8+ CTL in lymphoid organs in vivo and acquire the ability to directly stimulate T cell growth, division, and development of cytolytic activity in vitro. Our results thus establish a role for B7-H3 in the costimulation of T cell immune responses in vivo.

Original languageEnglish (US)
Pages (from-to)5445-5450
Number of pages6
JournalJournal of Immunology
Volume173
Issue number9
DOIs
StatePublished - Nov 1 2004
Externally publishedYes

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CD8 Antigens
Immunity
T-Lymphocytes
Neoplasms
B7 Antigens
Growth and Development
Cell Division
Transfection

ASJC Scopus subject areas

  • Immunology

Cite this

B7-H3 enhances tumor immunity in vivo by costimulating rapid clonal expansion of antigen-specific CD8+ cytolytic T cells. / Luo, Liqun; Chapoval, Andrei; Flies, Dallas B.; Zhu, Gefeng; Hirano, Fumiya; Wang, Shengdian; Lau, Julie S.; Dong, Haidong; Tamada, Koji; Flies, Andrew S.; Liu, Yang; Chen, Lieping.

In: Journal of Immunology, Vol. 173, No. 9, 01.11.2004, p. 5445-5450.

Research output: Contribution to journalArticle

Luo, L, Chapoval, A, Flies, DB, Zhu, G, Hirano, F, Wang, S, Lau, JS, Dong, H, Tamada, K, Flies, AS, Liu, Y & Chen, L 2004, 'B7-H3 enhances tumor immunity in vivo by costimulating rapid clonal expansion of antigen-specific CD8+ cytolytic T cells', Journal of Immunology, vol. 173, no. 9, pp. 5445-5450. https://doi.org/10.4049/jimmunol.173.9.5445
Luo, Liqun ; Chapoval, Andrei ; Flies, Dallas B. ; Zhu, Gefeng ; Hirano, Fumiya ; Wang, Shengdian ; Lau, Julie S. ; Dong, Haidong ; Tamada, Koji ; Flies, Andrew S. ; Liu, Yang ; Chen, Lieping. / B7-H3 enhances tumor immunity in vivo by costimulating rapid clonal expansion of antigen-specific CD8+ cytolytic T cells. In: Journal of Immunology. 2004 ; Vol. 173, No. 9. pp. 5445-5450.
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AU - Hirano, Fumiya

AU - Wang, Shengdian

AU - Lau, Julie S.

AU - Dong, Haidong

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