B7-H3: A costimulatory molecule for T cell activation and IFN-γ production

Andrei Chapoval, Jian Ni, Julie S. Lau, Ryan A. Wilcox, Dallas B. Flies, Ding Liu, Haidong Dong, Gabriel L. Sica, Gefeng Zhu, Koji Tamada, Lieping Chen

Research output: Contribution to journalArticle

511 Citations (Scopus)

Abstract

We describe here a newly identified member of the human B7 family, designated B7 homolog 3 (B7-H3), that shares 20-27% amino acid identity with other B7 family members. B7-H3 mRNA is not detectable in peripheral blood mononuclear cells, although it is found in various normal tissues and in several tumor cell lines. Expression of B7-H3 protein, however, can be induced on dendritic cells (DCs) and monocytes by inflammatory cytokines and a combination of phorbol myristate acetate (PMA) + ionomycin. Soluble B7-H3 protein binds a putative counter-receptor on activated T cells that is distinct from CD28, cytotoxic T lymphocyte antigen 4 (CTLA-4), inducible costimulator (ICOS) and PD-1. B7-H3 costimulates proliferation of both CD4+ and CD8+T cells, enhances the induction of cytotoxic T cells and selectively stimulates interferon γ (IFN-γ) production in the presence of T cell receptor signaling. In contrast, inclusion of antisense B7-H3 oligonucleotides decreases the expression of B7-H3 on DCs and inhibits IFN-γ production by DC-stimulated allogeneic T cells. Thus, we describe a newly identified costimulatory pathway that may participate in the regulation of cell-mediated immune responses.

Original languageEnglish (US)
Pages (from-to)269-274
Number of pages6
JournalNature Immunology
Volume2
Issue number3
DOIs
StatePublished - Mar 1 2001
Externally publishedYes

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Interferons
Dendritic Cells
T-Lymphocytes
Inducible T-Cell Co-Stimulator Protein
CTLA-4 Antigen
Ionomycin
Tetradecanoylphorbol Acetate
T-Cell Antigen Receptor
Tumor Cell Line
Oligonucleotides
Monocytes
Blood Cells
Proteins
Cytokines
Amino Acids
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Chapoval, A., Ni, J., Lau, J. S., Wilcox, R. A., Flies, D. B., Liu, D., ... Chen, L. (2001). B7-H3: A costimulatory molecule for T cell activation and IFN-γ production. Nature Immunology, 2(3), 269-274. https://doi.org/10.1038/85339

B7-H3 : A costimulatory molecule for T cell activation and IFN-γ production. / Chapoval, Andrei; Ni, Jian; Lau, Julie S.; Wilcox, Ryan A.; Flies, Dallas B.; Liu, Ding; Dong, Haidong; Sica, Gabriel L.; Zhu, Gefeng; Tamada, Koji; Chen, Lieping.

In: Nature Immunology, Vol. 2, No. 3, 01.03.2001, p. 269-274.

Research output: Contribution to journalArticle

Chapoval, A, Ni, J, Lau, JS, Wilcox, RA, Flies, DB, Liu, D, Dong, H, Sica, GL, Zhu, G, Tamada, K & Chen, L 2001, 'B7-H3: A costimulatory molecule for T cell activation and IFN-γ production', Nature Immunology, vol. 2, no. 3, pp. 269-274. https://doi.org/10.1038/85339
Chapoval, Andrei ; Ni, Jian ; Lau, Julie S. ; Wilcox, Ryan A. ; Flies, Dallas B. ; Liu, Ding ; Dong, Haidong ; Sica, Gabriel L. ; Zhu, Gefeng ; Tamada, Koji ; Chen, Lieping. / B7-H3 : A costimulatory molecule for T cell activation and IFN-γ production. In: Nature Immunology. 2001 ; Vol. 2, No. 3. pp. 269-274.
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