Abstract

Antimony (Sb), a toxic metalloid, is soluble as antimonate (Sb(V)). While bio-reduction of Sb(V) is an effective Sb-removal approach, its bio-reduction has been coupled to oxidation of only organic electron donors. In this study, we demonstrate, for the first time, the feasibility of autotrophic microbial Sb(V) reduction using hydrogen gas (H2) as the electron donor without extra organic carbon source. SEM and EDS analysis confirmed the production of the mineral precipitate Sb2O3. When H2 was utilized as the electron donor, the consortium was able to fully reduce 650 μM of Sb(V) to Sb(III) in 10 days, a rate comparable to the culture using lactate as the electron donor. The H2-fed culture directed a much larger fraction of it donor electrons to Sb(V) reduction than did the lactate-fed culture. While 98% of the electrons from H2 were used to reduce Sb(V) by the H2-fed culture, only 12% of the electrons from lactate was used to reduce Sb(V) by the lactate-fed culture. The rest of the electrons from lactate went to acetate and propionate through fermentation, to methane through methanogenesis, and to biomass synthesis. High-throughput sequencing confirmed that the microbial community for the lactate-fed culture was much more diverse than that for the H2-fed culture, which was dominated by a short rod-shaped phylotype of Rhizobium (α-Protobacteria) that may have been active in Sb(V) reduction.

Original languageEnglish (US)
Pages (from-to)467-474
Number of pages8
JournalWater Research
Volume88
DOIs
StatePublished - Jan 1 2016

Fingerprint

hydrogen
electron
Hydrogen
Electrons
Metalloids
antimony
methanogenesis
Antimony
rhizobacterium
Organic carbon
Fermentation
fermentation
Precipitates
Energy dispersive spectroscopy
microbial community
acetate
Methane
Biomass
Minerals
methane

Keywords

  • Antimonate reduction
  • Electron donor
  • Hydrogen
  • Rhizobium

ASJC Scopus subject areas

  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution
  • Ecological Modeling

Cite this

Lai, C. Y., Wen, L. L., Zhang, Y., Luo, S. S., Wang, Q. Y., Luo, Y. H., ... Zhao, H. P. (2016). Autotrophic antimonate bio-reduction using hydrogen as the electron donor. Water Research, 88, 467-474. https://doi.org/10.1016/j.watres.2015.10.042

Autotrophic antimonate bio-reduction using hydrogen as the electron donor. / Lai, Chun Yu; Wen, Li Lian; Zhang, Yin; Luo, Shan Shan; Wang, Qing Ying; Luo, Yi Hao; Chen, Ran; Yang, Xiaoe; Rittmann, Bruce; Zhao, He Ping.

In: Water Research, Vol. 88, 01.01.2016, p. 467-474.

Research output: Contribution to journalArticle

Lai, CY, Wen, LL, Zhang, Y, Luo, SS, Wang, QY, Luo, YH, Chen, R, Yang, X, Rittmann, B & Zhao, HP 2016, 'Autotrophic antimonate bio-reduction using hydrogen as the electron donor', Water Research, vol. 88, pp. 467-474. https://doi.org/10.1016/j.watres.2015.10.042
Lai, Chun Yu ; Wen, Li Lian ; Zhang, Yin ; Luo, Shan Shan ; Wang, Qing Ying ; Luo, Yi Hao ; Chen, Ran ; Yang, Xiaoe ; Rittmann, Bruce ; Zhao, He Ping. / Autotrophic antimonate bio-reduction using hydrogen as the electron donor. In: Water Research. 2016 ; Vol. 88. pp. 467-474.
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