Automation of yeast pedigree analysis

John Koschwanez, Mark Holl, Michael McMurray, Daniel Gottschling, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

Yeast pedigree analysis - isolation and characterization of the products of mitotic cell divisions throughout the lifespan of an individual cell - is a manually intensive process that requires a biologist to manipulate single yeast cells every 90 minutes for as long as 150 hours. Progress toward the development of a system for automating yeast pedigree analysis is presented. Yeast cells are trapped in a microfluidic device, then observed using a fiber-optic imaging bundle. Image processing software classifies each cell as either budding or not budding a newly formed daughter cell. The system recognizes when a cell has a bud, and microfluidic valves direct newly formed daughter cells to an agar plate. Finally, biologists analyze the colonies formed by each yeast daughter cell on the agar plate.

Original languageEnglish (US)
Title of host publicationProceedings - IEEE International Conference on Robotics and Automation
Pages1475-1480
Number of pages6
Volume2004
Edition2
StatePublished - 2004
Externally publishedYes
EventProceedings- 2004 IEEE International Conference on Robotics and Automation - New Orleans, LA, United States
Duration: Apr 26 2004May 1 2004

Other

OtherProceedings- 2004 IEEE International Conference on Robotics and Automation
CountryUnited States
CityNew Orleans, LA
Period4/26/045/1/04

Keywords

  • Automation
  • Genomics
  • Imaging
  • Microfluidics
  • Yeast

ASJC Scopus subject areas

  • Software
  • Control and Systems Engineering

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  • Cite this

    Koschwanez, J., Holl, M., McMurray, M., Gottschling, D., & Meldrum, D. (2004). Automation of yeast pedigree analysis. In Proceedings - IEEE International Conference on Robotics and Automation (2 ed., Vol. 2004, pp. 1475-1480)