Abstract
Yeast pedigree analysis - isolation and characterization of the products of mitotic cell divisions throughout the lifespan of an individual cell - is a manually intensive process that requires a biologist to manipulate single yeast cells every 90 minutes for as long as 150 hours. Progress toward the development of a system for automating yeast pedigree analysis is presented. Yeast cells are trapped in a microfluidic device, then observed using a fiber-optic imaging bundle. Image processing software classifies each cell as either budding or not budding a newly formed daughter cell. The system recognizes when a cell has a bud, and microfluidic valves direct newly formed daughter cells to an agar plate. Finally, biologists analyze the colonies formed by each yeast daughter cell on the agar plate.
| Original language | English (US) |
|---|---|
| Title of host publication | Proceedings - IEEE International Conference on Robotics and Automation |
| Pages | 1475-1480 |
| Number of pages | 6 |
| Volume | 2004 |
| Edition | 2 |
| State | Published - 2004 |
| Externally published | Yes |
| Event | Proceedings- 2004 IEEE International Conference on Robotics and Automation - New Orleans, LA, United States Duration: Apr 26 2004 → May 1 2004 |
Other
| Other | Proceedings- 2004 IEEE International Conference on Robotics and Automation |
|---|---|
| Country/Territory | United States |
| City | New Orleans, LA |
| Period | 4/26/04 → 5/1/04 |
Keywords
- Automation
- Genomics
- Imaging
- Microfluidics
- Yeast
ASJC Scopus subject areas
- Software
- Control and Systems Engineering