Automated vision-based selection and placement of single cells in microwell array formats

Yasser H. Anis, Mark R. Holl, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

16 Scopus citations

Abstract

A robotic manipulation system for automated selection and transfer of individual living cells of interest to analysis locations is presented. The system is the first in a series of operational components comprising an integrated system for the analysis of cell expression and metabolism at the single cell level. In our approach, we begin with a commonly used cell transfer technique, which uses glass capillary micropipettes to aspirate and release cells. Using a vision-based feedback closed-loop control process, two individual three-axis robotic stages are used to position the micropipette tip in proximity to the cell of interest. The cell is aspirated and the tip is moved to a target location, where the cell is then dispensed. For our initial application, the target location for living cells of interest is a microwell containing sensors that measure metabolic parameters. The system, when complete with fully integrated functionality, will offer a robust and flexible technology for cell selection and manipulation. Applications for this technology include precise tools for multi-parameter analysis of single cells, cell patterning, tissue engineering, and cell surgery.

Original languageEnglish (US)
Title of host publication4th IEEE Conference on Automation Science and Engineering, CASE 2008
Pages315-320
Number of pages6
DOIs
StatePublished - Nov 3 2008
Event4th IEEE Conference on Automation Science and Engineering, CASE 2008 - Washington, DC, United States
Duration: Aug 23 2008Aug 26 2008

Publication series

Name4th IEEE Conference on Automation Science and Engineering, CASE 2008

Other

Other4th IEEE Conference on Automation Science and Engineering, CASE 2008
CountryUnited States
CityWashington, DC
Period8/23/088/26/08

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Anis, Y. H., Holl, M. R., & Meldrum, D. (2008). Automated vision-based selection and placement of single cells in microwell array formats. In 4th IEEE Conference on Automation Science and Engineering, CASE 2008 (pp. 315-320). [4626575] (4th IEEE Conference on Automation Science and Engineering, CASE 2008). https://doi.org/10.1109/COASE.2008.4626575