Automated vision-based selection and placement of single cells in microwell array formats

Yasser H. Anis, Mark R. Holl, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

17 Scopus citations

Abstract

A robotic manipulation system for automated selection and transfer of individual living cells of interest to analysis locations is presented. The system is the first in a series of operational components comprising an integrated system for the analysis of cell expression and metabolism at the single cell level. In our approach, we begin with a commonly used cell transfer technique, which uses glass capillary micropipettes to aspirate and release cells. Using a vision-based feedback closed-loop control process, two individual three-axis robotic stages are used to position the micropipette tip in proximity to the cell of interest. The cell is aspirated and the tip is moved to a target location, where the cell is then dispensed. For our initial application, the target location for living cells of interest is a microwell containing sensors that measure metabolic parameters. The system, when complete with fully integrated functionality, will offer a robust and flexible technology for cell selection and manipulation. Applications for this technology include precise tools for multi-parameter analysis of single cells, cell patterning, tissue engineering, and cell surgery.

Original languageEnglish (US)
Title of host publication4th IEEE Conference on Automation Science and Engineering, CASE 2008
Pages315-320
Number of pages6
DOIs
StatePublished - Nov 3 2008
Event4th IEEE Conference on Automation Science and Engineering, CASE 2008 - Washington, DC, United States
Duration: Aug 23 2008Aug 26 2008

Publication series

Name4th IEEE Conference on Automation Science and Engineering, CASE 2008

Other

Other4th IEEE Conference on Automation Science and Engineering, CASE 2008
Country/TerritoryUnited States
CityWashington, DC
Period8/23/088/26/08

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

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