Automated solid-phase synthesis of high capacity oligo-dT cellulose for affinity purification of poly-A tagged biomolecules

Sujay P. Sau, Andrew C. Larsen, John C. Chaput

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Affinity purification of poly-adenylated biomolecules using solid supports that are derivatized with poly-thymidine oligonucleotides provides a powerful method for isolating cellular mRNA. These systems have also been used to purify mRNA-peptide fusions generated by RNA-display. However, the commercial source for high capacity oligo-dT cellulose was recently discontinued. To overcome this problem, we have developed a low cost solid-phase synthesis protocol to generate oligo-dT cellulose. Comparative binding studies indicate that chemically synthesized oligo-dT cellulose functions with superior loading capacity when compared to the discontinued product. We suggest that this method could be used to synthesize oligo-dT resin for routine purification of poly-adenylated biomolecules.

Original languageEnglish (US)
Pages (from-to)5692-5694
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume24
Issue number24
DOIs
StatePublished - Dec 15 2014

Fingerprint

Solid-Phase Synthesis Techniques
Poly A
Biomolecules
Purification
Messenger RNA
Oligonucleotides
Thymidine
Fusion reactions
Resins
Display devices
RNA
Costs and Cost Analysis
Peptides
oligo(dT)-cellulose
Costs

Keywords

  • Affinity purification
  • mRNA display
  • Oligo-dT cellulose

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Molecular Medicine
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

Automated solid-phase synthesis of high capacity oligo-dT cellulose for affinity purification of poly-A tagged biomolecules. / Sau, Sujay P.; Larsen, Andrew C.; Chaput, John C.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 24, No. 24, 15.12.2014, p. 5692-5694.

Research output: Contribution to journalArticle

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