Abstract
We present a robotic manipulation system for automated selection and transfer of individual living cells to analysis locations. We begin with a commonly used cell transfer technique using glass capillary micropipettes to aspirate and release living cells suspended in liquid growth media. Using vision-based feedback and closed-loop process control, two individual three-axis robotic stages position the micropipette tip in proximity to the cell of interest. The cell is aspirated and the tip is moved to a target location where the cell is dispensed. Computer vision is used to monitor and inspect the success of the dispensing process. In our initial application, the target cell destination is a microwell etched in a fused silica substrate. The system offers a robust and flexible technology for cell selection and manipulation. Applications for this technology include embryonic stem cells transfer, blastomere biopsy, cell patterning, and cell surgery.
Original language | English (US) |
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Article number | 5373841 |
Pages (from-to) | 598-606 |
Number of pages | 9 |
Journal | IEEE Transactions on Automation Science and Engineering |
Volume | 7 |
Issue number | 3 |
DOIs | |
State | Published - Jul 2010 |
Keywords
- Automation
- cell manipulation
- closed-loop control
- feature recognition
- image processing
- visual servoing
ASJC Scopus subject areas
- Control and Systems Engineering
- Electrical and Electronic Engineering