Automated digital light modulation microscope (DLMM) for living cell array analysis: Pattern recognition and spatial alignment

Shih Hui Chao, Timothy T.H. Ren, Steven A. Gales, Mark R. Holl, Sarah C. McQuaide, Deirdre R. Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

The Microscale Life Sciences Center at the University of Washington is developing a suite of technologies that combine high-density cell assays and automatic platforms to achieve high-content living cell screening [1]. The use of microfluidic cell arrays allows for the manipulation of cells and control of biochemical stimuli in parallel, making automated operation possible. However, screening the microscale cell arrays is still slow because the current imaging techniques can only observe cells sequentially and lacks feedback to control light in the interrogating regions of interest (ROI). In this paper, we report using a digital light modulation microscope (DLMM) that observes cells in the arrays in parallel so the throughput can increase dramatically. We have developed and present an algorithm to align the light modulator to the imager and to determine the ROI automatically.

Original languageEnglish (US)
Title of host publicationProceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006
Pages977-981
Number of pages5
DOIs
StatePublished - Dec 22 2006
Externally publishedYes
Event1st IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006 - Pisa, Italy
Duration: Feb 20 2006Feb 22 2006

Publication series

NameProceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006
Volume2006

Other

Other1st IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006
CountryItaly
CityPisa
Period2/20/062/22/06

Keywords

  • DMD
  • Digital light modulation microscope
  • High content cell screening
  • Large-scale cell array
  • Microfluidics

ASJC Scopus subject areas

  • Engineering(all)

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    Chao, S. H., Ren, T. T. H., Gales, S. A., Holl, M. R., McQuaide, S. C., & Meldrum, D. R. (2006). Automated digital light modulation microscope (DLMM) for living cell array analysis: Pattern recognition and spatial alignment. In Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006 (pp. 977-981). [1639218] (Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006; Vol. 2006). https://doi.org/10.1109/BIOROB.2006.1639218