Auracyanin, a Blue Copper Protein from the Green Photosynthetic Bacterium Chloroflexus aurantiacus

Jeffrey T. Trost; James D. McManus; John C. Freeman; B. L. Ramakrishna; Robert E. Blankenship

doi:10.1021/bi00420a041

Auracyanin, a Blue Copper Protein from the Green Photosynthetic Bacterium Chloroflexus aurantiacus

Jeffrey T. Trost, James D. McManus, John C. Freeman, B. L. Ramakrishna, Robert E. Blankenship

Engineering of Matter, Transport and Energy, School for (IAFSE-SEMTE)

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Abstract

: A small, type 1 blue copper protein has been isolated from the green photosynthetic bacterium Chloroflexus aurantiacus. This protein, named auracyanin, appears to be peripherally associated with the cytoplasmic membrane, with a midpoint potential of +240 mV and a molar extinction coefficient of e₅₉₆= 2900 M^-1cm^-1. Auracyanin is isolated as a disulfide-bridged dimer with a monomer molecular mass of 12 800 Da. The isoelectric point of auracyanin is 4.0. ESR spectra exhibit rhombic distortion and give no indication of interaction between the coppers. The function of auracyanin is not yet known, although its redox properties are compatible with a role in photosynthetic and/or respiratory electron flow.

Original language	English (US)
Pages (from-to)	7858-7863
Number of pages	6
Journal	Biochemistry
Volume	27
Issue number	20
DOIs	https://doi.org/10.1021/bi00420a041
State	Published - Oct 1 1988

ASJC Scopus subject areas

Biochemistry

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title = "Auracyanin, a Blue Copper Protein from the Green Photosynthetic Bacterium Chloroflexus aurantiacus",

abstract = ": A small, type 1 blue copper protein has been isolated from the green photosynthetic bacterium Chloroflexus aurantiacus. This protein, named auracyanin, appears to be peripherally associated with the cytoplasmic membrane, with a midpoint potential of +240 mV and a molar extinction coefficient of e596= 2900 M-1cm-1. Auracyanin is isolated as a disulfide-bridged dimer with a monomer molecular mass of 12 800 Da. The isoelectric point of auracyanin is 4.0. ESR spectra exhibit rhombic distortion and give no indication of interaction between the coppers. The function of auracyanin is not yet known, although its redox properties are compatible with a role in photosynthetic and/or respiratory electron flow.",

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T1 - Auracyanin, a Blue Copper Protein from the Green Photosynthetic Bacterium Chloroflexus aurantiacus

AU - Trost, Jeffrey T.

AU - McManus, James D.

AU - Freeman, John C.

AU - Ramakrishna, B. L.

AU - Blankenship, Robert E.

PY - 1988/10/1

Y1 - 1988/10/1

N2 - : A small, type 1 blue copper protein has been isolated from the green photosynthetic bacterium Chloroflexus aurantiacus. This protein, named auracyanin, appears to be peripherally associated with the cytoplasmic membrane, with a midpoint potential of +240 mV and a molar extinction coefficient of e596= 2900 M-1cm-1. Auracyanin is isolated as a disulfide-bridged dimer with a monomer molecular mass of 12 800 Da. The isoelectric point of auracyanin is 4.0. ESR spectra exhibit rhombic distortion and give no indication of interaction between the coppers. The function of auracyanin is not yet known, although its redox properties are compatible with a role in photosynthetic and/or respiratory electron flow.

AB - : A small, type 1 blue copper protein has been isolated from the green photosynthetic bacterium Chloroflexus aurantiacus. This protein, named auracyanin, appears to be peripherally associated with the cytoplasmic membrane, with a midpoint potential of +240 mV and a molar extinction coefficient of e596= 2900 M-1cm-1. Auracyanin is isolated as a disulfide-bridged dimer with a monomer molecular mass of 12 800 Da. The isoelectric point of auracyanin is 4.0. ESR spectra exhibit rhombic distortion and give no indication of interaction between the coppers. The function of auracyanin is not yet known, although its redox properties are compatible with a role in photosynthetic and/or respiratory electron flow.

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Auracyanin, a Blue Copper Protein from the Green Photosynthetic Bacterium Chloroflexus aurantiacus

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