AuNP-DG: Deoxyglucose-labeled gold nanoparticles as X-ray computed tomography contrast agents for cancer imaging

Bulent Aydogan, Ji Li, Tijana Rajh, Ahmed Chaudhary, Steven J. Chmura, Charles Pelizzari, Christian Wietholt, Metin Kurtoglu, Peter Redmond

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

Purpose: To study the feasibility of using 2-deoxy-d-glucose (2-DG)-labeled gold nanoparticle (AuNP-DG) as a computed tomography (CT) contrast agent with tumor targeting capability through in vitro experiments. Procedures: Gold nanoparticles (AuNP) were fabricated and were conjugated with 2-deoxy-d-glucose. The human alveolar epithelial cancer cell line, A-549, was chosen for the in vitro cellular uptake assay. Two groups of cell samples were incubated with the AuNP-DG and the unlabeled AuNP, respectively. Following the incubation, the cells were washed with sterile PBS to remove the excess gold nanoparticles and spun to cell pellets using a centrifuge. The cell pellets were imaged using a microCT scanner immediately after the centrifugation. The reconstructed CT images were analyzed using a commercial software package. Results: Significant contrast enhancement in the cell samples incubated with the AuNP-DG with respect to the cell samples incubated with the unlabeled AuNP was observed in multiple CT slices. Conclusions: Results from this study demonstrate enhanced uptake of 2-DG-labeled gold nanoparticle by cancer cells in vitro and warrant further experiments to study the exact molecular mechanism by which the AuNP-DG is internalized and retained in the tumor cells.

Original languageEnglish (US)
Pages (from-to)463-467
Number of pages5
JournalMolecular Imaging and Biology
Volume12
Issue number5
DOIs
StatePublished - Oct 2010
Externally publishedYes

Keywords

  • 2-Deoxy-d-glucose
  • Cancer imaging
  • Contrast-enhanced CT
  • Gold nanoparticle
  • Tumor targeting

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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